Increased staining for phospho-Akt, p65-RELA and cIAP-2 in pre-neoplastic human bronchial biopsiesReport as inadecuate

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BMC Cancer

, 5:155

First Online: 06 December 2005Received: 15 August 2005Accepted: 06 December 2005DOI: 10.1186-1471-2407-5-155

Cite this article as: Tichelaar, J.W., Zhang, Y., leRiche, J.C. et al. BMC Cancer 2005 5: 155. doi:10.1186-1471-2407-5-155


BackgroundThe development of non-small cell lung carcinoma proceeds through a series of well-defined pathological steps before the appearance of invasive lung carcinoma. The molecular changes that correspond with pathology changes are not well defined and identification of the molecular events may provide clues on the progression of intraepithelial neoplasia in the lung, as well as suggest potential targets for chemoprevention. The acquisition of anti-apoptotic signals is critical for the survival of cancer cells but the pathways involved are incompletely characterized in developing intra-epithelial neoplasia IEN.

MethodsWe used immunohistochemistry to determine the presence, relative levels, and localization of proteins that mediate anti-apoptotic pathways in developing human bronchial neoplasia.

ResultsBronchial epithelial protein levels of the phosphorylated active form of AKT kinase and the caspase inhibitor cIAP-2 were increased in more advanced grades of bronchial IEN lesions than in normal bronchial epithelium. Additionally, the percentage of biopsies with nuclear localization of p65-RELA in epithelial cells increased with advancing pathology grade, suggesting that NF-κB transcriptional activity was induced more frequently in advanced IEN lesions.

ConclusionOur results indicate that anti-apoptotic pathways are elevated in bronchial IEN lesions prior to the onset of invasive carcinoma and that targeting these pathways therapeutically may offer promise in prevention of non-small cell lung carcinoma.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-5-155 contains supplementary material, which is available to authorized users.

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Author: Jay W Tichelaar - Yu Zhang - Jean C leRiche - Paul W Biddinger - Stephen Lam - Marshall W Anderson


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