Shin’iseihaito Xinyiqingfeitang Suppresses the Biofilm Formation of Streptococcus pneumoniae In VitroReport as inadecuate




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BioMed Research International - Volume 2017 2017, Article ID 4575709, 8 pages - https:-doi.org-10.1155-2017-4575709

Research Article

Department of Bacteriology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-ku, Nagoya, Japan

Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-Dori, Mizuho-ku, Nagoya, Japan

Core Laboratory, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan

Correspondence should be addressed to Masaaki Minami

Received 13 February 2017; Accepted 5 April 2017; Published 16 April 2017

Academic Editor: Kong Chen

Copyright © 2017 Masaaki Minami et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Streptococcus pneumoniae S. pneumoniae is the important pathogen that causes otolaryngeal diseases such as sinusitis. S. pneumoniae frequently forms the biofilm to prevent severe circumstances such as antimicrobial agents. Shin’iseihaito xinyiqingfeitang is a formula of Japanese traditional Kampo medicine that has 9 crude drugs and provides the medicinal usage for sinusitis. The objective of the present study is to reveal the mechanism of antibiofilm activity by Shin’iseihaito extract SSHT. SSHT significantly inhibited the formation of biofilm from S. pneumoniae ATCC 49619 in dose- and time-dependent manners. SSHT also significantly suppressed the biofilm formation by other five different cps types of S. pneumoniae clinical isolates. We found that the extracts of 8 out of 9 components in Shin’iseihaito had the inhibitory effects of biofilm formation, and the extract of the root of Scutellaria baicalensis had the strongest effect among the ingredients of Shin’iseihaito. We found that the capsule of SSHT-treated S. pneumoniae was significantly thinner than that of the untreated group and that SSHT reduced the hydrophobicity of bacterial cell surface. Our results suggest that Shin’iseihaito may be a useful agent for the treatment of S. pneumoniae-induced sinusitis because of the inhibition of biofilm formation of S. pneumoniae.





Author: Masaaki Minami, Toru Konishi, Hiroshi Takase, and Toshiaki Makino

Source: https://www.hindawi.com/



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