CD8 , HLA-unrestricted, cytotoxic T-lymphocyte line against malignant melanomaReport as inadecuate




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Journal of Translational Medicine

, 3:41

First Online: 10 November 2005Received: 27 October 2005Accepted: 10 November 2005DOI: 10.1186-1479-5876-3-41

Cite this article as: Somasundaram, R., Caputo, L., Guerry, D. et al. J Transl Med 2005 3: 41. doi:10.1186-1479-5876-3-41

Abstract

A CD8 cytotoxic T lymphocyte CTL line was derived from the peripheral blood mononuclear cells of a patient with primary melanoma. The CD8 CTL line specifically lysed the autologous primary melanoma cells and not the natural killer cell-sensitive K562 cells or lymphokine activated killer cell-sensitive DAUDI cells. When a large panel of human leukocyte antigen HLA-matched and -unmatched allogeneic melanoma, glioma, breast and colorectal carcinoma cells was tested as targets in cytolysis assays, 4 HLA-matched and two HLA-unmatched allogeneic metastatic melanoma lines were lysed by the CD8 CTL. Lysis of autologous and allogeneic melanoma cells was dependent on the effector-to-target cell ratio. Lysis of autologous melanoma cells was not blocked by anti-HLA class I or class II antibodies, confirming that the cytolytic activity of the CD8 CTL was HLA-unrestricted. CTL lysis of autologous melanoma cells was CD3 T cell receptor dependent and FAS-FAS-L, and CD1 independent. Identification of the melanoma-associated antigen recognized by the HLA-unrestricted CTL may provide a vaccine for a broad population of melanoma patients.

List of abbreviationsCTLcytotoxic T cell

EBVEpstein-Barr virus

E:Teffector-to-target

FITCfluorescein isothiocyanate

GM-CSFgranulocyte monocyte-colony stimulating factor

HLAhuman leukocyte antigen

IFN-γinterferon gamma

ILinterleukin

LAKlymphokine-actived killer

MAbmonoclonal antibody

MLTCmixed lymphocyte tumor culture

MUC1mammary-pancreas mucin

PBMCperipheral blood mononuclear cells

PEphycoerythrin

RIAradioimmunoassay

RT-PCRreverse transcriptase-polymerase chain reaction

TCRT-cell receptor

TNFtumor necrosis factor.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-3-41 contains supplementary material, which is available to authorized users.

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Author: Rajasekharan Somasundaram - Laura Caputo - DuPont Guerry - Dorothee Herlyn

Source: https://link.springer.com/







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