Diffusion-weighted and dynamic contrast-enhanced MRI of pancreatic adenocarcinoma xenografts: associations with tumor differentiation and collagen contentReport as inadecuate




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Journal of Translational Medicine

, 14:161

First Online: 07 June 2016Received: 25 February 2016Accepted: 20 May 2016DOI: 10.1186-s12967-016-0920-y

Cite this article as: Wegner, C.S., Gaustad, JV., Andersen, L.M.K. et al. J Transl Med 2016 14: 161. doi:10.1186-s12967-016-0920-y

Abstract

PurposeThe aggressiveness of pancreatic ductal adenocarcinoma PDAC is highly dependent on the level of differentiation and the composition of the stroma. In this preclinical study, we investigated the potential of diffusion-weighted magnetic resonance imaging DW-MRI and dynamic contrast-enhanced magnetic resonance imaging DCE-MRI as noninvasive methods for providing information on the differentiation and the stroma of PDACs.

MethodsXenografted tumors initiated from four PDAC cell lines BxPC-3, Capan-2, MIAPaCa-2, and Panc-1 were included in the study. DW-MRI and DCE-MRI were carried out on a 7.05-T MR scanner, and tumor images of ADC the apparent diffusion coefficient, K the volume transfer constant of Gd-DOTA, and ve the fractional distribution volume of Gd-DOTA were produced. The level of differentiation and the amount and structure of collagen I and collagen IV were determined by examining histological preparations.

ResultsDifferentiated tumors showed lower levels of collagen I and collagen IV than non-differentiated tumors. Significant correlations were found between ADC and ve, and both parameters differentiated clearly between collagen-rich non-differentiated tumors and differentiated tumors containing less collagen.

ConclusionDifferentiated PDAC xenografts show higher ADC values and higher ve values than their non-differentiated counterparts. This observation supports the application of parametric MR images as tumor biomarkers in PDAC. Patients showing low values of ADC and ve most likely have non-differentiated tumors with extensive stroma and, hence, poor prognosis.

KeywordsPancreatic ductal adenocarcinoma Magnetic resonance imaging Parametric MR images Differentiation of PDAC Stroma of PDAC Electronic supplementary materialThe online version of this article doi:10.1186-s12967-016-0920-y contains supplementary material, which is available to authorized users.

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Author: Catherine S. Wegner - Jon-Vidar Gaustad - Lise Mari K. Andersen - Trude G. Simonsen - Einar K. Rofstad

Source: https://link.springer.com/







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