Characterization of a Murine Anti-laminin-1 Monoclonal Antibody AK8 Produced by Immunization with Mouse-derived Laminin-1Report as inadecuate

Characterization of a Murine Anti-laminin-1 Monoclonal Antibody AK8 Produced by Immunization with Mouse-derived Laminin-1 - Download this document for free, or read online. Document in PDF available to download.

Clinical and Developmental Immunology - Volume 12 2005, Issue 1, Pages 67-73

Department of Obstetrics and Gynecology, Center for Specialized Clinical Science, Tokai University, School of Medicine, Ishehara, Japan

Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry, Shikata-cho, 2-5-1Shikata-cho, Okayama 700-8558, Japan

Department of Laboratory for Molecular Science Research, Tokai University, School of Medicine, Ishehara, Japan

Biomedical Computation Center, Osaka Medical College, Takatsuki, Japan

Department of Obstetrics and Gynecology, Okayama University Hospital, Okayama, Japan

Tokyo University of Pharmacy and Life Science, Hachiouji, Japan

Shiseido Life Science Research Center, Yokohama, Japan

Corgenix Inc, Westminster, Colorado, USA

Department of Medicine B and Center for Autoimmune Diseases, Sheba Medical Center, Tel-hashomer, Israel

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a significant association between anti-laminin-1 antibodies Abs and reproductive failure, such as recurrent spontaneous abortions and infertility-associated endometriosis in both human and mouse studies. In the present study, we established an IgM μ,κ monoclonal anti-laminin-1 Ab AK8 by immunizing mice with mouse Engelbreth-Holm-Swarm sarcoma EHS-derived laminin-α1. The AK8 monoclonal antibody mAb reacted with particular peptide sequences from the globular G domain of mouse laminin-α1 chain of using ELISA and Western blot techniques. The peptide tertiary structure of the epitope recognized by AK8 mAb was predicted using eight synthesized domain peptide sequences and three consensus sequences obtained by phage displayed random peptide library. Basement membranes of endometrium of pregnant mice and humans were immunostained with AK8 mAb. Thus, AK8 mAb recognized a common structure present in the G domain of the laminin-1 chain in both mice and humans. The passive immunization of mice with AK8 mAb may represent a suitable animal model for anti-laminin-1 Ab-mediated reproductive failure.

Author: Akane Kondo, Junko Inagaki, Kazuko Kobayashi, Hideo Tsukamoto, Daisuke Yamamoto, Mikiya Nakatsuka, Nobuharu Suzuki, Motoyoshi Nom



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