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Case Reports in Ophthalmological Medicine - Volume 2016 2016, Article ID 4148968, 4 pages -

Case Report

Department of Ophthalmology, Ross Eye Institute, The State University of New York at Buffalo, Jacobs School of Medicine and Biomedical Sciences, 1176 Main Street, Buffalo, NY 14209, USA

SUNY Eye Institute, Buffalo, NY 14214, USA

Research Service, Veterans Administration Western New York Healthcare System VAWNYHS, Building 20, 3495 Bailey Avenue, Buffalo, NY 14215, USA

Department of Pathology, Buffalo General Medical Center, Kaleida Health, 100 High Street, Buffalo, NY 14203, USA

Department of Pathology, The State University of New York at Buffalo, Jacobs School of Medicine and Biomedical Sciences, 206 Farber Hall, Buffalo, NY 14214, USA

Departments of Ophthalmology and Pathology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA

Research and Development Service, G.V. Sonny Montgomery Veterans Affair Medical Center, 1500 East Woodrow Wilson Avenue, Jackson, MS 39216, USA

Received 7 January 2016; Accepted 17 May 2016

Academic Editor: Dipak Parmar

Copyright © 2016 Sangita P. Patel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. Acanthamoeba keratitis remains a difficult diagnosis despite advances in genetic and imaging technologies. The purpose of this paper is to highlight the utility of cytology smears for diagnosis of Acanthamoeba keratitis. Methods. This is a case study of the diagnostic course for a patient with suspected Acanthamoeba keratitis. Results. A 40-year-old male with poor contact lens hygiene presented with severe left eye pain. Slit lamp examination showed two peripheral ring infiltrates without an epithelial defect. The epithelium over both infiltrates was removed with a Kimura spatula. Half of the sample was smeared on a dry microscope slide and the other half was submitted for Acanthamoeba culture and PCR. Both culture and PCR were negative for Acanthamoeba, but hematoxylin and eosin stain of the smear revealed double-walled cysts. Conclusion. HandE staining of corneal cytology specimens is an efficient and readily available test for diagnosis of Acanthamoeba keratitis.

Autor: Sangita P. Patel, Jamie L. Schaefer, Ryan Jaber, Joyce Paterson, Weiguo Liu, and Federico Gonzalez-Fernandez



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