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Journal of Translational Medicine

, 2:10

First Online: 07 April 2004Received: 31 January 2004Accepted: 07 April 2004DOI: 10.1186-1479-5876-2-10

Cite this article as: Li, F., Yang, X.Y., Jiang, W.H. et al. J Transl Med 2004 2: 10. doi:10.1186-1479-5876-2-10

Abstract

Nasopharyngeal carcinoma NPC is one of the most commons cancers in Southeast Asia and Southern China. Several NPC-associated genes have been so far described and here we describe the identification and the characterization of a novel nasopharyngeal carcinoma-associated peptide: NAP-1. NAP-1 was identified with the human genome draft searching method combined with nested PCR mapping of the chromosome 4q13 region. NAP-1 encodes an 85 amino acid alkaline peptide with a calculated isoelectric point of 9.3, three phosphorilation sites and a proline-rich region. Northern blot analysis revealed that NAP-1 is expressed as a 0.6 kb transcript in normal lymph nodes and trachea. In addition, reverse transcription RT-PCR showed that NAP-1 is expressed not only in NPC but in normal nasopharynx NP and various other tumors and tissues of the head and neck including: tonsils, lymph nodes, carcinoma of the tonsil, T cell lymphomas, squamous cell carcinoma of the hard palate, papilloma of the nasopharynx, nasopharyngitis, lymphoma of the tongue root and follicular dendritic cells FDC. In addition, NAP-1 is not expressed in normal tissues or tumors from other anatomical regions and was not expressed by NPC cell lines. Surprisingly, differential RT-PCR demonstrated decreased expression of NAP-1 in NPC compared with paired NP biopsies in 42.5 % of cases 17 out of 40. In addition, in situ hybridization and immunohistochemistry demonstrated that NAP-1 is expressed by S100 CD35 FDCs of the germinal center and not in other normal immune cells infiltrating NP or NPC. Therefore, it is likely that NAP-1 is secreted by FDC in the NP and may play an immune modulatory role in NPC.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-2-10 contains supplementary material, which is available to authorized users.





Autor: Feng Li - Xu Y Yang - Wei H Jiang - Zhi H Yin - Xiang L Feng - Wei D Liu - Lei Wang - Wen Zhou - Cai P Ren - Kai T

Fuente: https://link.springer.com/







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