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BMC Cardiovascular Disorders

, 2:8

First Online: 05 May 2002Received: 17 February 2002Accepted: 05 May 2002DOI: 10.1186-1471-2261-2-8

Cite this article as: Alm, R., Edvinsson, L. & Malmsjö, M. BMC Cardiovasc Disord 2002 2: 8. doi:10.1186-1471-2261-2-8


BackgroundEndothelium dysfunction is believed to play a role in the development of cardiovascular disease. The aim of the present study was to evaluate the suitability of organ culture as a model for endothelium dysfunction.

MethodsThe isometric tension was recorded in isolated segments of the rat mesenteric artery branch, before and after organ culture for 20 h. Vasodilatation was expressed as % of preconstriction with U46619. The acetylcholine ACh induced nitric oxide NO mediated dilatation was studied in the presence of 10 μM indomethacin, 50 nM charybdotoxin and 1 μM apamin. Endothelium-derived hyperpolarising factor EDHF was studied in the presence of 0.1 mM L-NOARG and indomethacin. Prostaglandins were studied in the presence of L-NOARG, charybdotoxin and apamin.

ResultsThe ACh-induced NO and prostaglandin-mediated dilatations decreased significantly during organ culture NO: 84% in control and 36% in cultured; prostaglandins: 48% in control and 16% in cultured. Notably, the total ACh-dilatation was not changed. This might be explained by the finding that EDHF alone stimulated a full dilatation even after organ culture 83% in control and 80% in cultured. EDHF may thereby compensate for the loss in NO and prostaglandin-mediated dilatation. Dilatations induced by forskolin or sodium nitroprusside did not change after organ culture, indicating intact smooth muscle cell function.

ConclusionsOrgan culture induces a loss in NO and prostaglandin-mediated dilatation, which is compensated for by EDHF. This shift in mediator profile resembles that in endothelium dysfunction. Organ culture provides an easily accessible model where the molecular changes that take place, when endothelium dysfunction is developed, can be examined over time.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2261-2-8 contains supplementary material, which is available to authorized users.

Autor: Rikard Alm - Lars Edvinsson - Malin Malmsjö


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