Expression of p53, inducible nitric oxide synthase and vascular endothelial growth factor in gastric precancerous and cancerous lesions: correlation with clinical featuresReport as inadecuate

Expression of p53, inducible nitric oxide synthase and vascular endothelial growth factor in gastric precancerous and cancerous lesions: correlation with clinical features - Download this document for free, or read online. Document in PDF available to download.

BMC Cancer

, 2:8

First Online: 29 April 2002Received: 06 November 2001Accepted: 29 April 2002DOI: 10.1186-1471-2407-2-8

Cite this article as: Feng, C.W., Wang, L.D., Jiao, L.H. et al. BMC Cancer 2002 2: 8. doi:10.1186-1471-2407-2-8


BackgroundThe growth and metastasis of tumors depend on the development of an adequate blood supply via angiogenesis. Recent studies indicate that the inducible nitric oxide synthase iNOS, vascular endothelial growth factor VEGF and the tumor suppressor p53 are fundamental play-markers of the angiogenic process. Overexpression of iNOS and VEGF has been shown to induce angiogenesis in tumors. P53 suppresses angiogenesis by down-regulating VEGF and iNOS. The correlation of expression of p53, VEGF and iNOS and clinical features in gastric carcinogenesis, however, has not been well characterized.

MethodsThe expression of p53, iNOS and VEGF in gastric precancerous and cancerous lesions and its relation with the clinical features was determined with immunohistochemistry avidin-biotin-peroxidase complex method on 55 randomly selected GC patients and 60 symptom-free subjects from the mass survey in the high-incidence area for GC in Henan, northern China.

ResultsThe positive immunostainig rates for p53, iNOS and VEGF in gastric carcinomas were 51%, 44% and 51%, respectively, and correlated well with TNM stages, but did not show significant difference among the groups with different degrees of gastric wall invasion depth by GC. A positive immunostaining reaction for the iNOS protein was significantly correlated with lymph node metastasis p = 0.019; Spearman correlation coefficient. P53 protein accumulation was higher in the poorly-differentiated gastric carcinoma than in well-differentiated one. In gastric biopsies, no positive immunosatining was observed for p53, iNOS and VEGF in the histologically normal tissue and chronic superficial gastritis CSG. However, p53, iNOS and VEGF positive immunostaining was observed in the tissues with different severities of lesions of chronic atrophic gastritis CAG, intestinal metaplasia IM and dysplasia DYS, and the positive rates increased with the lesion progression from CAG to IM to DYS. A high coincidental positive and negative immunostaining rate for p53, iNOS and VEGF was observed both in biopsy samples with CAG, IM and DYS from the symptom-free subjects and in gastric cancer tissue from the GC patients.

ConclusionsThe present results indicated that p53 protein accumulation and increased expression of iNOS and VEGF might be responsible for gastric carcinogenesis and tumor aggressiveness of gastric cancer.

AbbreviationsThe abbreviations used areCSG, chronic superficial gastritis

CAGchronic atrophic gastritis


GCgastric adenocarcinoma

iNOSinducible nitric oxide synthase

IMintestinal metaplasia

VEGFvascular endothelial growth factor.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-2-8 contains supplementary material, which is available to authorized users.

Author: Chang Wei Feng - Li Dong Wang - Lian Hua Jiao - Bin Liu - Shu Zheng - Xin Ji Xie


Related documents