The effect of lipoaspirates vs. dissected abdominal fat on breast cancer cells in vitroReportar como inadecuado




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European Journal of Medical Research

, 22:10

First Online: 21 March 2017Received: 12 September 2016Accepted: 07 March 2017DOI: 10.1186-s40001-017-0251-3

Cite this article as: Almarzouqi, F., Rennekampff, HO., Stromps, JP. et al. Eur J Med Res 2017 22: 10. doi:10.1186-s40001-017-0251-3

Abstract

BackgroundCancer cells are typically surrounded by stromal cells and embedded in extracellular matrix ECM. The stromal compartment interacts with cancer cells to promote growth and metastasis. For decades, autologous fasciocutaneous flaps have been safely applied for breast reconstruction after mastectomy. In contrast, the safety of fat grafting lipofilling procedure has been under debate regarding the risk of cancer recurrence.

MethodsHarvested fat tissue lipoaspirates and dissected abdominal fat DAF were co-cultured with MCF-7 breast cancer cells. The vitality of MCF-7 cells was measured using AlamarBlue consecutively for 5 days. ECM degradation was determined by detection of matrix metalloproteinase-1 MMP-1 expression in MCF-7 cells. Integrin α2 was measured by Western blot to assess the degree of adhesion and motility of MFC-7 cells.

ResultsThe MCF-7 proliferation increased substantially when co-cultured with fat tissue. However, there was no significant difference between the proliferation stimulating effects of lipoaspirates and DAF. Similarly, MMP-1 protein expression was equally elevated in MCF-7 cells by both lipoaspirates and DAF. Importantly, MCF-7 cells showed an increased level of integrin α2 once co-cultured with either lipoaspirates or DAF.

ConclusionFat tissue increases the proliferation of MCF-7 cells in vitro. Our data suggest that lipoaspirates as well as DAF might possess a considerable potency to promote tumorigenic growth of breast cancer cells. Thus, clinical trials are needed to address the safety of lipofilling by breast reconstruction surgery after mastectomy.

KeywordsFat grafting Breast cancer Breast reconstruction AbbreviationsASPSAmerican Society of Plastic Surgeons

ASCsadipose-derived Stem cells

bFGFbasic fibroblast growth factor

BMEbasement membrane extract

BMIbody mass index

DAFdissected abdominal fat

DIEP flapdeep inferior epigastric artery perforator flap

ECMextracellular matrix

FCSfetal calf serum

IGF-1insulin-like growth factor 1

MMPsmatrix metalloproteinases

msTRAM flapmuscle-sparing Transvers Rectus Abdominis Myocutaneous flap

NEAAnon-essential amino acids

PDGF-BBplatelet-derived growth factor

RPMI 1640 mediumRoswell Park Memorial Institute medium

SEMstandard error of mean

VEGFvascular endothelial growth factor





Autor: Faris Almarzouqi - Hans-Oliver Rennekampff - Jan-Philipp Stromps - Ziyad Alharbi - Norbert Pallua

Fuente: https://link.springer.com/







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