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International Journal of Bacteriology - Volume 2014 2014, Article ID 795281, 7 pages -

Research Article

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street S.W., Rochester, MN 55905, USA

Mayo High School, 1420 11th Avenue S.E., Rochester, MN 55904, USA

Division of Infectious Disease, Department of Medicine, Mayo Clinic, 200 First Streer S.W., Rochester, MN 55905, USA

Received 17 November 2013; Revised 9 January 2014; Accepted 23 January 2014; Published 26 February 2014

Academic Editor: Mariagrazia Perilli

Copyright © 2014 Michelle E. M. Campeau and Robin Patel. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We assessed the in vitro activity of Manuka honey against biofilm bacteria in combination with antibiotics and visualized the effect of Manuka honey on bacterial biofilms using scanning electron microscopy. The fractional biofilm eradication concentration index for vancomycin plus Manuka honey against S. aureus IDRL-4284 biofilms was 0.34, indicating a synergistic interaction. The index for gentamicin plus Manuka honey against P. aeruginosa PAO1 biofilms was 0.78–0.82, indicating an additive interaction. Scanning electron microscopy of S. aureus IDRL-4284 and P. aeruginosa PAO1 biofilms exposed to Manuka honey and artificial honey containing the same sugar composition as Manuka honey showed that the former had more pronounced effects than the latter on both S. aureus and P. aeruginosa biofilms. Visualized effects included distorted cell morphologies for both bacteria and a decrease in P. aeruginosa extracellular matrix. In conclusion, Manuka honey has a synergistic interaction with vancomycin against S. aureus biofilms and an additive interaction with gentamicin against P. aeruginosa biofilms.

Autor: Michelle E. M. Campeau and Robin Patel



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