Cell–Cell Interaction of Macrophages and Vascular Smooth Muscle Cells in the Synthesis of Leukotriene B4Reportar como inadecuado

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Mediators of Inflammation - Volume 3 1994, Issue 4, Pages 297-302

Laboratoire d-lmmunopharmacologie, Institut des Cordeliers, 15 rue de I-école de Mddecine, Paris 75270, France

Laboratoire de Biochimie, Hôpital Broussais, Paris 75674, France

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Biosynthesis of LTB4 during cell-cell interaction betweenvascular smooth muscle cells SMC and alveolar macrophages AM hasbeen investigated by use of both high-pressure Hquid chromatographyHPLC and radtoimmunoassay RIA. Both interleukin-βIL-β and tumour necrosis factor-α TNFα induceda time- and dose-dependent synthesis of 15-, and5-hydroxyeicosatetraenoic acids HETEs from cultured SMC. However,neither TNFα nor IL-1β induced a significantLTB4 production in SMC alone or AM alone after 24 h ofincubation. Addition of IL-1β and TNFα simultaneously toSMC resulted in a dose-dependent synergistic increase of HETEs.Macrophages dose-dependently transformed extremely lowconcentrations of exogenous LTA4 into LTB4.Incubation of vascular SMC with various numbers of AM in thepresence of IL-1β 5 units-ml and TNFα 10units-ml induced a great increase of LTB4 synthesisin comparison with the detectable levels of LTB4 producedby macrophages alone. Pretreatment of SMC with NDGA, cycloheximide,and actinomycin not only inhibited IL-1 and TNT induced HETEssynthesis but also abolished LTB4 production whenco-incubated with macrophages. These results suggest thatLTB4 in a mixture of SMC and macrophages could originatefrom a transcellular metabolism, i.e. macrophages transformingSMC-derived LTA4 into LTB4.

Autor: M. Zou and C. Anges

Fuente: https://www.hindawi.com/


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