Differential phenotypes of memory CD4 and CD8 T cells in the spleen and peripheral tissues following immunostimulatory therapyReportar como inadecuado




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Journal for ImmunoTherapy of Cancer

, 5:33

Immunotherapy Biomarkers

Abstract

BackgroundStudies assessing immune parameters typically utilize human PBMCs or murine splenocytes to generate data that is interpreted as representative of immune status. Using splenocytes, we have shown memory CD4-T cells that expand following systemic immunostimulatory therapies undergo rapid IFNg-mediated activation induced cell death AICD resulting in a net loss of total CD4-T cells which correlates with elevated PD-1 expression. This is in contrast to CD8-T cells which expand with minimal PD-1 upregulation and apoptosis. In this study we expand upon our previous work by evaluating CD4 and CD8-T cell phenotype and distribution in peripheral organs which are more representative of immune responses occurring at metastatic sites following immunotherapy.

MethodsPhenotypic assessment of T cells in both lymphoid spleen and LN as well as peripheral organs liver and lungs in control and immunotherapy treated mice was performed to survey the impact of location on memory phenotype and activation marker status. Peripheral blood from patients undergoing systemic high dose IL-2 was also assessed for expression of PD-1 and memory phenotype.

ResultsHere we reveal that, similar to what occurs in the spleen and lymph nodes, CD4-T cell numbers decreased while CD8-T cells expanded at these peripheral sites. In contrast to having differential expression of PD-1 as occurs in the spleen, both CD4 and CD8-T cells had significantly elevated levels of PD-1 in both the liver and lungs. Further analysis correlated PD-1 expression to CD62L T effector-effector memory,TE-EM expression which are more prevalent in CD4-T cells in general as well as CD8-T cells in peripheral organs. Similar elevated PD-1 expression on TE-EM cells was observed in patients undergoing systemic high-dose IL-2 therapy.

ConclusionsThese data highlight PD-1 expressing and-or TE-EM subsets of T cells in circulation as more representative of cells at immune sites and underscore the importance of valuation both in lymphoid as well as target organs when making determinations about immune status.

Trial registrationClinicalTrials.gov NCT01416831. Registered August 12, 2011.

KeywordsImmunotherapy Cancer PD-1 Bystander Activation CD8 NKG2D AbbreviationsBrdUBromodeoxyuridine

Foxp3Forkhead box p3

IFNgInterferon gamma

IL-2Interleukin-2

ITImmunotherapy

IUInternational units

KgKilogram

KLRG1Killer cell lectin like receptor subfamily G member 1

LNLymph node

MHCMajor histocompatibility complex

MIN-OMammary intraepithelial neoplasia outgrowth

NKG2DNatural killer group 2D

PBMCsPeripheral blood mononuclear cells

PD-1Programmed death 1

SPFSpecific pathogen free

TCMcentral memory T cell

TCRT cell receptor

TE-EMeffector-effector memory T cell

TLRToll like receptor

Electronic supplementary materialThe online version of this article doi:10.1186-s40425-017-0235-4 contains supplementary material, which is available to authorized users.





Autor: Gail D. Sckisel - Annie Mirsoian - Christine M. Minnar - Marka Crittenden - Brendan Curti - Jane Q. Chen - Bruce R. Bla

Fuente: https://link.springer.com/







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