Growth and Replication of Infectious Bursal Disease Virus in the DF-1 Cell Line and Chicken Embryo FibroblastsReportar como inadecuado

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BioMed Research International - Volume 2014 2014, Article ID 494835, 6 pages -

Research Article

Department of Environmental and Herbal Science, Tamil University, Thanjavur, Tamil Nadu 613005, India

Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 143701, Republic of Korea

Received 26 February 2014; Accepted 3 May 2014; Published 14 May 2014

Academic Editor: Peirong Jiao

Copyright © 2014 Kaliyaperumal Rekha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Infectious bursal disease virus IBDV causes a highly contagious disease in young chicks and leads to significant economic losses in the poultry industry. To determine a suitable cell line for IBDV infection, replication, and growth kinetics of the virus, DF-1 cells and chicken embryo fibroblasts CEF were used. The population doubling per day Pd-D was found to be higher in DF-1 as compared to CEF cells. A suitable time of infection TOI was established for increased production of virus and greater infectivity titers. The DF-1 and CEF cells were found to be susceptible to infection by producing marked cytopathic effects CPEs, and the growth curves of IBDV in DF-1 and CEF cells were evaluated by infectivity assay using tissue culture infectious dose TCID50. The cytopathic effects of the virus in DF-1 and CEF cells were found to be similar, but higher viral titers were detected in the DF-1 cells as compared to CEF. Thus the DF-1 cell line had a higher growth potential and infectivity, which will be of advantage in vaccine production.

Autor: Kaliyaperumal Rekha, Chandran Sivasubramanian, Ill-Min Chung, and Muthu Thiruvengadam



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