Transgenic Carrot Expressing Fusion Protein Comprising M. tuberculosis Antigens Induces Immune Response in MiceReport as inadecuate

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BioMed Research International - Volume 2015 2015, Article ID 417565, 11 pages -

Research Article

Institute of Cytology and Genetics, Russian Academy of Sciences, Prospect Lavrentieva 10, Novosibirsk 630090, Russia

State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk 630559, Russia

Received 13 January 2015; Accepted 20 March 2015

Academic Editor: Pengjun Shi

Copyright © 2015 Natalia V. Permyakova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Tuberculosis remains one of the major infectious diseases, which continues to pose a major global health problem. Transgenic plants may serve as bioreactors to produce heterologous proteins including antibodies, antigens, and hormones. In the present study, a genetic construct has been designed that comprises the Mycobacterium tuberculosis genes cfp10, esat6 and dIFN gene, which encode deltaferon, a recombinant analog of the human γ-interferon designed for expression in plant tissues. This construct was transferred to the carrot Daucus carota L. genome by Agrobacterium-mediated transformation. This study demonstrates that the fusion protein CFP10-ESAT6-dIFN is synthesized in the transgenic carrot storage roots. The protein is able to induce both humoral and cell-mediated immune responses in laboratory animals mice when administered either orally or by injection. It should be emphasized that M. tuberculosis antigens contained in the fusion protein have no cytotoxic effect on peripheral blood mononuclear cells.

Author: Natalia V. Permyakova, Alla A. Zagorskaya, Pavel A. Belavin, Elena A. Uvarova, Olesya V. Nosareva, Andrey E. Nesterov, Anna



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