Upregulation of heme oxygenase-1 expression by curcumin conferring protection from hydrogen peroxide-induced apoptosis in H9c2 cardiomyoblastsReportar como inadecuado




Upregulation of heme oxygenase-1 expression by curcumin conferring protection from hydrogen peroxide-induced apoptosis in H9c2 cardiomyoblasts - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Cell and Bioscience

, 7:20

First Online: 21 April 2017Received: 05 January 2017Accepted: 19 April 2017DOI: 10.1186-s13578-017-0146-6

Cite this article as: Yang, X., Jiang, H. & Shi, Y. Cell Biosci 2017 7: 20. doi:10.1186-s13578-017-0146-6

Abstract

BackgroundCurcumin is a major constituent of rhizomes of Curcuma longa that elicits beneficial effects for oxidative damage. The aim of this study was to investigate whether curcumin could attenuate hydrogen peroxide H2O2-induced apoptosis in H9c2 cardiomyoblasts and the underlying mechanisms.

ResultsThe present study showed that exposure of H9c2 cells to H2O2 caused a significant increase in apoptosis as evaluated by flow cytometry analysis and the pretreatment of curcumin protected against H2O2-induced apoptosis. Exposure of cells with curcumin caused a dose-dependent induction of heme oxygenase-1 HO-1 protein expression. Curcumin also decreased the cleaved caspase-3 CC3 protein expression level and increased the Bcl-2-Bax ratio in H2O2-stimulated H9c2 cells. ZnPP-IX, a HO-1 inhibitor, partly reversed the anti-apoptotic effect of curcumin. Further, LY294002, an inhibitor of PI3K, partially reversed the effect of curcumin on HO-1 protein induction, leading to the attenuation of curcumin-mediated apoptosis resistance.

ConclusionThese results demonstrated that the anti-apoptotic function of curcumin required the upregulation of HO-1 protein through the PI3K-Akt signaling pathway. Curcumin might be used as a preventive and therapeutic agent for treatment of cardiovascular diseases associated with oxidative stress.

KeywordsCurcumin Cardiomyocyte apoptosis Oxidative stress Heme oxygenase-1 PI3K-Akt AbbreviationsH2O2hydrogen peroxide

HO-1heme oxygenase-1

CC3cleaved caspase-3

ROSreactive oxygen species

COcarbon monoxide

PKBprotein kinase B

PI3 Kphosphoinositide 3-kinase

ERKsextracellular signal-regulated kinases

MTTmethyl thiazolyl tetrazolium

ZnPP-IXZine protoporphyrin-IX

DMSOdimethyl sulfoxide

ATCCAmerican Type Culture Collection





Autor: Xiaobo Yang - Hong Jiang - Yao Shi

Fuente: https://link.springer.com/







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