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International Journal of ElectrochemistryVolume 2011 2011, Article ID 823734, 6 pages

Research Article

Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India

Bio-MEMS and Microsystem Lab, Department of Electrical Engineering, University of South Florida, Tampa, FL 33620, USA

Department of Engineering Sciences and Materials, University of Puerto Rico, Mayaguez, PR 00680, USA

Received 14 April 2011; Accepted 17 August 2011

Academic Editor: Hassan Ali Zamani

Copyright © 2011 Shibu Saha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A novel hybrid matrix of zinc oxide-methylene blue ZnO-MB has been successfully developed for biosensing application. The introduction of methylene blue into the ZnO thin film leads to reduction in the charge transfer resistance and suggests an increase in the electron transfer capacity of the composite. Glucose oxidase GOx was chosen as the model enzyme and effectively immobilized on the surface of hybrid ZnO-MB nanocomposite matrix. Electrochemical measurements were employed to study biosensing response of the GOx-ZnO-MB-ITO bioelectrode as a function of glucose concentration. The low oxidation potential −0.23 V of the hybrid bioelectrode, in a mediatorless electrolyte, makes it resistant against interference from other bio-molecules. The low value of Michaelis-Menten constant 2.65 mM indicates that immobilized GOx retains its enzymatic activity significantly on the surface of nanocomposite hybrid matrix that results in an enhanced affinity towards its substrate glucose. The ZnO-MB nanocomposite hybrid matrix, exhibiting enhanced sensing response 0.2 μAmM


with long shelf-life >10 weeks, has potential for the realization of an integrated biosensing device.

Autor: Shibu Saha, S. K. Arya, S. P. Singh, and Vinay Gupta

Fuente: https://www.hindawi.com/


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