11C-L-methyl methionine dynamic PET-CT of skeletal muscle: response to protein supplementation compared to L-ring 13C6 phenylalanine infusion with serial muscle biopsyReportar como inadecuado




11C-L-methyl methionine dynamic PET-CT of skeletal muscle: response to protein supplementation compared to L-ring 13C6 phenylalanine infusion with serial muscle biopsy - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Annals of Nuclear Medicine

, Volume 31, Issue 4, pp 295–303

First Online: 04 March 2017Received: 02 September 2016Accepted: 01 February 2017DOI: 10.1007-s12149-017-1157-4

Cite this article as: Arentson-Lantz, E.J., Saeed, I.H., Frassetto, L.A. et al. Ann Nucl Med 2017 31: 295. doi:10.1007-s12149-017-1157-4

Abstract

ObjectiveThe objective of this study was to determine if clinical dynamic PET-CT imaging with C-L-methyl-methionine C-MET in healthy older women can provide an estimate of tissue-level post-absorptive and post-prandial skeletal muscle protein synthesis that is consistent with the more traditional method of calculating fractional synthesis rate FSR of muscle protein synthesis from skeletal muscle biopsies obtained during an infusion of L-ring C6 phenylalanine C6-Phe.

MethodsHealthy older women 73 ± 5 years completed both dynamic PET-CT imaging with C-MET and a stable isotope infusion of C6-Phe with biopsies to measure the skeletal muscle protein synthetic response to 25 g of a whey protein supplement. Graphical estimation of the Patlak coefficient Ki from analysis of the dynamic PET-CT images was employed as a measure of incorporation of 11 C-MET in the mid-thigh muscle bundle.

ResultsPost-prandial values mean ± standard error of the mean SEM were higher than post-absorptive values for both Ki 0.0095 ± 0.001 vs. 0.00785 ± 0.001 min, p < 0.05 and FSR 0.083 ± 0.008 vs. 0.049 ± 0.006%-h, p < 0.001 in response to the whey protein supplement. The percent increase in Ki and FSR in response to the whey protein supplement was significantly correlated r = 0.79, p = 0.015.

ConclusionsDynamic PET-CT imaging with C-MET provides an estimate of the post-prandial anabolic response that is consistent with a traditional, invasive stable isotope, and muscle biopsy approach. These results support the potential future use of C-MET imaging as a non-invasive method for assessing conditions affecting skeletal muscle protein synthesis.

KeywordsPET-CT Human Muscle protein synthesis FSR AbbreviationsFSRFractional synthetic rate

KiPatlak Coefficient, representing fractional extraction rate Ki from plasma into a peripheral compartment bound protein

C-PheL- ring-C6 phenylalanine

PETPositron emission tomography

C-METC-L-methyl-methionine

MPSMuscle protein synthesis

PSRProtein synthetic rate

CTComputed tomography

IDIFImage-derived input function

TACTissue activity curve

CAActivity of 11 C-MET measured in femoral artery by IDIF analysis

CTActivity of 11 C-MET measured in skeletal muscle tissue at each point in the TAC

E. J. Arentson-Lantz and I. H. Saeed are co-first authors.





Autor: Emily J. Arentson-Lantz - Isra H. Saeed - Lynda A. Frassetto - Umesh Masharani - Roy J. Harnish - Youngho Seo - Henry F

Fuente: https://link.springer.com/







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