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carassius-auratus, expression, cloning, NMR structure, mice, neuropeptide-y, in-situ hybridization, NPY messenger-RNA, bass dicentrarchus-labrax, receptor

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Subject-Keyword: carassius-auratus expression cloning NMR structure mice neuropeptide-y in-situ hybridization NPY messenger-RNA bass dicentrarchus-labrax receptor

Type of item: Journal Article Published

Language: English

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Description: Agouti-related protein AGRP is a naturally occurring antagonist of melanocortin. In mammals, central AGRP expression is restricted to the arcuate nucleus in which it plays a key role in the control of energy balance by antagonizing melanocortin effects at melanocortin 4 receptors. In goldfish, melanocortin 4 receptor is profusely expressed within the main brain areas for the control of energy balance, and central administration of agonist or antagonist analogs inhibits or stimulates food intake, respectively. Here we demonstrate that the goldfish genome has a homologous gene to mammalian AGRP. Detailed brain mapping by in situ hybridization shows that AGRP is exclusively expressed in the ventrobasal hypothalamic lateral tuberal nucleus, the teleostean homolog of the arcuate nucleus. Fasting up-regulates its mRNA levels in the lateral tuberal nucleus. In the periphery, AGRP is expressed in several tissues including ovary, muscle, and ventral skin, suggesting that AGRP might regulate peripheral actions of melanocortin peptides. The results provide the first evidence for an endogenous melanocortin antagonist in nontetrapod species and suggest that hypothalamic overexpression during fasting might regulate the inhibitory effects of melanocortin peptides on food intake in goldfish.

Date created: 2003

DOI: doi:10.7939-R3BN9X55R

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Rights: Copyright © 2003 by The Endocrine Society





Autor: Cerdá-Reverter, J.M. Peter, R.E.

Fuente: https://era.library.ualberta.ca/


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0013-7227-03-$15.00-0 Printed in U.S.A. Endocrinology 144(10):4552– 4561 Copyright © 2003 by The Endocrine Society doi: 10.1210-en.2003-0453 Endogenous Melanocortin Antagonist in Fish: Structure, Brain Mapping, and Regulation by Fasting of the Goldfish Agouti-Related Protein Gene JOSÉ MIGUEL CERDÁ-REVERTER AND RICHARD ECTOR PETER Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9 Agouti-related protein (AGRP) is a naturally occurring antagonist of melanocortin.
In mammals, central AGRP expression is restricted to the arcuate nucleus in which it plays a key role in the control of energy balance by antagonizing melanocortin effects at melanocortin 4 receptors.
In goldfish, melanocortin 4 receptor is profusely expressed within the main brain areas for the control of energy balance, and central administration of agonist or antagonist analogs inhibits or stimulates food intake, respectively.
Here we demonstrate that the goldfish genome has a homologous gene to mammalian AGRP.
Detailed brain mapping by in situ hybridization shows that AGRP is exclusively expressed in the ventrobasal V ARIOUS EVIDENCE INDICATES that central melanocortin signaling is a nodal point in the control of energy balance in mammals by exerting an inhibitory tone on food intake and stimulating energy expenditure (1, 2).
Melanocortin signaling is mediated by binding to a family of specific G protein-coupled receptors that positively couple to adenylyl cyclase.
Five melanocortin receptors (MC1RMC5R) have been characterized by molecular cloning in mammals and chicken.
Peptides derived from proopiomelanocortin (POMC), namely ACTH and melanocyte-stimulating hormones (MSH), ␣-, ␤-, and ␥-MSH, are the main known agonists for MCRs, whereas agouti and agoutirelated protein (AGRP) act as endogenous antagonists (3). Agouti protein is a potent melanocortin antagonist at MC1R and MC4R (4), whereas AGRP is inactive at MC1R but equally potent in inhibiti...





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