IGF-1 modulates gene expression of proteins involved in inflammation, cytoskeleton, and liver architectureReport as inadecuate

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Journal of Physiology and Biochemistry

, Volume 73, Issue 2, pp 245–258

First Online: 26 January 2017Received: 18 April 2016Accepted: 16 December 2016DOI: 10.1007-s13105-016-0545-x

Cite this article as: Lara-Diaz, V., Castilla-Cortazar, I., Martín-Estal, I. et al. J Physiol Biochem 2017 73: 245. doi:10.1007-s13105-016-0545-x


Even though the liver synthesizes most of circulating IGF-1, it lacks its receptor under physiological conditions. However, according to previous studies, a damaged liver expresses the receptor. For this reason, herein, we examine hepatic histology and expression of genes encoding proteins of the cytoskeleton, extracellular matrix, and cell-cell molecules and inflammation-related proteins. A partial IGF-1 deficiency murine model was used to investigate IGF-1’s effects on liver by comparing wild-type controls, heterozygous igf1, and heterozygous mice treated with IGF-1 for 10 days. Histology, microarray for mRNA gene expression, RT-qPCR, and lipid peroxidation were assessed. Microarray analyses revealed significant underexpression of igf1 in heterozygous mice compared to control mice, restoring normal liver expression after treatment, which then normalized its circulating levels. IGF-1 receptor mRNA was overexpressed in Hz mice liver, while treated mice displayed a similar expression to that of the controls. Heterozygous mice showed overexpression of several genes encoding proteins related to inflammatory and acute-phase proteins and underexpression or overexpression of genes which coded for extracellular matrix, cytoskeleton, and cell junction components. Histology revealed an altered hepatic architecture. In addition, liver oxidative damage was found increased in the heterozygous group. The mere IGF-1 partial deficiency is associated with relevant alterations of the hepatic architecture and expression of genes involved in cytoskeleton, hepatocyte polarity, cell junctions, and extracellular matrix proteins. Moreover, it induces hepatic expression of the IGF-1 receptor and elevated acute-phase and inflammation mediators, which all resulted in liver oxidative damage.

KeywordsIGF-1 Gene expression Cytoskeleton Tight junctions Hepatocytes Extracellular matrix AbbreviationsANOVAAnalysis of variance


CTcycle threshold

ECMExtracellular matrix

ELISAEnzyme-linked immunosorbent assay

GHGrowth hormone

HClHydrochloric acid

HandEHematoxylin and Eosin

HLAHuman leucocyte antigen

HzHeterozygous igf-1

IGFBPsIGF-1 binding proteins

IGF-1Insulin-like growth factor-1

IGF-1RIGF-1 receptor

IUGRintrauterine growth restriction



mRNAMessenger ribonucleic acid

PBSPhosphate buffer saline

PCRPolymerase chain reaction

RT-qPCRReal-time quantitative polymerase chain reaction

SEMStandard error of mean

SPSSStatistical Package for the Social Sciences

TGFβTransforming growth factor β

WTWild-type, control group

Lara-Diaz VJ and Castilla-Cortázar I contributed equally to this work

Electronic supplementary materialThe online version of this article doi:10.1007-s13105-016-0545-x contains supplementary material, which is available to authorized users.

Author: VJ Lara-Diaz - I Castilla-Cortazar - I Martín-Estal - M García-Magariño - GA Aguirre - JE Puche - RG de la Garza - LA 

Source: https://link.springer.com/

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