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Genes and Genomics

, Volume 39, Issue 5, pp 533–540

First Online: 09 February 2017Received: 09 November 2016Accepted: 24 January 2017DOI: 10.1007-s13258-017-0518-7

Cite this article as: Bong, I.P.N., Ng, C.C., Baharuddin, P. et al. Genes Genom 2017 39: 533. doi:10.1007-s13258-017-0518-7

Abstract

Epigenetic changes have emerged as key causes in the development and progression of multiple myeloma MM. In this study, global microRNA miRNA expression profiling were performed for 27 MM 19 specimens and 8 cell lines and 3 normal controls by microarray. miRNA-targets were identified by integrating the miRNA expression profiles with mRNA expression profiles of the matched samples unpublished data. Two miRNAs were selected for verification by RT-qPCR miR-150-5p and miR-4430. A total of 1791 and 8 miRNAs were over-expressed and under-expressed, respectively in MM compared to the controls fold change ≥2.0; p < 0.05. The miRNA-mRNA integrative analysis revealed inverse correlation between 5 putative target genes RAD54L, CCNA2, CYSLTR2, RASGRF2 and HKDC1 and 15 miRNAs p < 0.05. Most of the differentially expressed miRNAs are involved in survival, proliferation, migration, invasion and drug resistance in MM. Some have never been described in association with MM miR-33a, miR-9 and miR-211. Interestingly, our results revealed 2 miRNAs, which are closely related to B cell differentiation miR-150 and miR-125b. For the first time, we suggest that miR-150 might be potential negative regulator for two critical cell cycle control genes, RAD54L and CCNA2, whereas miR-125b potentially target RAS and CysLT signaling proteins, namely RASGRF2 and CYSLTR2, respectively. This study has enhanced our understanding on the pathobiology of MM and opens up new avenues for future research in myelomagenesis.

KeywordsMultiple myeloma MicroRNA Microarray MiRNA-mRNA integrative analysis MiR-150 MiR-125b Electronic supplementary materialThe online version of this article doi:10.1007-s13258-017-0518-7 contains supplementary material, which is available to authorized users.





Autor: Ivyna Pau Ni Bong - Ching Ching Ng - Puteri Baharuddin - Zubaidah Zakaria

Fuente: https://link.springer.com/







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