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The Scientific World Journal - Volume 2014 2014, Article ID 285405, 6 pages -

Research Article

Moscow State Academy of Veterinary Medicine and Biotechnology, Akad. Skryabin Street 23, Moscow 109472, Russia

Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia

Received 22 July 2014; Accepted 2 September 2014; Published 14 October 2014

Academic Editor: Ya Cheng

Copyright © 2014 Sergei Yu. Zaitsev et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Conjugates of the photoactivated rhodamine dyes with biopolymers proteins, polysaccharides, and nucleic acids are important tools for microscopic investigation of biological tissue. In this study, a precursor of the photoactivated fluorescent dye PFD has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan “Chitosan-PFD” and histone H1 “Histone H1.3-PFD”. The intensive fluorescence has been observed after photoactivation of these conjugates inside cells A431, HaCaT, HEK239, HBL-100, and MDCK. Developed procedures and obtained data are important for further application of novel precursors of fluorescent dyes “caged” dyes for microscopic probing of biological objects. Thus, the synthesized “Chitosan-PFD” and “Histone H1-PFD” have been successfully applied in this study for intracellular transport visualization by fluorescent microscopy.





Autor: Sergei Yu. Zaitsev, Mikhail N. Shaposhnikov, Daria O. Solovyeva, Valeria V. Solovyeva, and Albert A. Rizvanov

Fuente: https://www.hindawi.com/



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