Astragalus Polysaccharide Protects Astrocytes from Being Infected by HSV-1 through TLR3-NF- B Signaling PathwayReportar como inadecuado




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Evidence-Based Complementary and Alternative MedicineVolume 2014 2014, Article ID 285356, 6 pages

Research Article

Department of Pharmacology, Weifang Medical University, 7166 Baotong West Street, Weifang 261053, China

Hospital of Weifang Medical University, 7166 Baotong West Street, Weifang 261053, China

The Affiliated Hospital of Shandong Traditional Chinese Medicine Junior College, 508 Binhai East Road, Yantai 264199, China

Department of Parasitology, Weifang Medical University, 7166 Baotong West Street, Weifang 261053, China

Received 17 April 2014; Revised 10 June 2014; Accepted 12 June 2014; Published 26 June 2014

Academic Editor: Shi-Biao Wu

Copyright © 2014 Lihong Shi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Astragalus polysaccharide APS is the most immunoreactive substance in Astragalus. APS can regulate the body’s immunity and is widely used in many immune related diseases. However, till now, there is little information about its contribution to the protection of astrocytes infected by virus. Toll-like receptor 3 TLR3 is a key component of the innate immune system and has the ability to detect virus infection and trigger host defence responses. This study was undertaken to elucidate the protective effect of APS on herpes simplex virus HSV-1 infected astrocytes and the underlying mechanisms. The results showed that APS protected the astrocytes from HSV-1 induced proliferation inhibition along with increasing expression of tumor necrosis factor-α TNF-α and interleukin-6 IL-6 markedly. Moreover, APS significantly promoted the expression of Toll-like receptor 3 TLR3 and the activation of nuclear factor-κB NF-κB in astrocytes. In addition, while astrocytes were pretreated with TLR3 antibody before adding HSV-1 and APS, the expression of TLR3, TNF-α, and IL-6 and the activation of NF-κB decreased sharply. These results indicate that APS can protect astrocytes by promoting immunological function provoked by HSV-1 through TLR3-NF-κB pathway.





Autor: Lihong Shi, Fengling Yin, Xiangui Xin, Shumei Mao, Pingping Hu, Chunzhen Zhao, and Xiuning Sun

Fuente: https://www.hindawi.com/



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