In Vitro Assessment of Cadmium Bioavailability in Chinese Cabbage Grown on Different Soils and Its Toxic Effects on Human HealthReport as inadecuate

In Vitro Assessment of Cadmium Bioavailability in Chinese Cabbage Grown on Different Soils and Its Toxic Effects on Human Health - Download this document for free, or read online. Document in PDF available to download.

BioMed Research International - Volume 2015 2015, Article ID 285351, 12 pages -

Research Article

Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China

Department of Environmental Science, International Islamic University, Islamabad 44000, Pakistan

Indian River Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Fort Pierce, FL 34945, USA

Department of Thyroid and Breast Surgery, Zhejiang Provincial People’s Hospital, Hangzhou 310014, China

Received 1 January 2015; Accepted 21 January 2015

Academic Editor: Qaisar Mahmood

Copyright © 2015 Rukhsanda Aziz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The minimum concentration of cadmium Cd, by Chinese cabbage grown on Cd contaminated soils that can initiate toxicity in human liver cells using in vitro digestion coupled with Caco-2-HL-7702 cell models was studied. Cadmium bioaccessibility in the gastric phase for yellow soil YS cabbage 40.84% and calcareous soil CS cabbage 21.54% was significantly higher than small intestinal phase with the corresponding values of 21.2% and 11.11%, respectively. Cadmium bioavailability was higher in YS cabbage 5.27%–14.66% than in CS cabbage 1.12%–9.64%. Cadmium concentrations >0.74 μg transported from YS and CS cabbage were able to induce oxidative MDA, H2O2 stress by inhibiting antioxidant SOD, GPx enzyme activities in human liver cells HL-7702. Additionally the study revealed that the ingestion of Cd contaminated Chinese cabbage grown in acidic soil yellow soil weakened the antioxidant defense system under all levels of contamination 2, 6, and 9 mg·kg

which ultimately escalated the oxidative stress in liver cells; however, in case of CS cabbage, a marked oxidative stress was observed only at 9 mg kg

Cd level of soil. Therefore, it is necessary to monitor Cd concentrations in leafy vegetables grown on acidic soils to minimize human health risk.

Author: Rukhsanda Aziz, Muhammad Tariq Rafiq, Zhenli He, Di Liu, Kewang Sun, and Yang Xiaoe



Related documents