Significantly enhanced biomass production of a novel bio-therapeutic strain Lactobacillus plantarum AS-14 by developing low cost media cultivation strategyReportar como inadecuado

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Journal of Biological Engineering

, 11:17

First Online: 05 May 2017Received: 19 November 2016Accepted: 05 April 2017DOI: 10.1186-s13036-017-0059-2

Cite this article as: Manzoor, A., Qazi, J.I., Haq, I. et al. J Biol Eng 2017 11: 17. doi:10.1186-s13036-017-0059-2


BackgroundProbiotic bacteria are becoming an important tool for improving human health, controlling diseases and enhancing immune responses. The availability of a cost effective cultivation conditions has profound effect on the efficiency and role of probiotic bacteria. Therefore the current study was conducted with an objective to develop a low cost growth medium for enhancing the biomass production of a bio-therapeutic bacterial strain Lactobacillus plantarum AS-14. In this work the isolation of Lactobacillus plantarum AS-14 bacterial strain was carried out from brinjal using cheese whey as a main carbon source. Moreover, the effect of four other nutritional factors besides cheese whey was investigated on the enhanced cell mass production by using response surface methodology RSM.

ResultsThe best culture medium contained 60 g-l cheese whey, 15 g-l glucose and 15 g-l corn steep liquor in addition to other minor ingredients and it resulted in maximum dry cell mass 15.41 g-l. The second-order polynomial regression model determined that the maximum cell mass production 16.02 g-l would be obtained at temperature 40°C and pH 6.2. Comparative studies showed that cultivation using cheese whey and corn steep liquor with other components of the selected medium generated higher biomass with lower cost than that of De Man, Rogosa and Sharpe MRS medium under similar cultivation conditions pH 6.2 and temperature 40°C.

ConclusionIt is evident that the cell biomass of L. Plantarum AS-14 was enhanced by low cost cultivation conditions. Moreover, corn steep liquor and ammonium bisulphate were perceived as low-cost nitrogen sources in combination with other components to substitute yeast extract. Of all these factors, cheese whey, corn steep liquor, yeast extract and two operating conditions temperature and pH were found to be the most significant parameters. Thus the cost effective medium developed in this research might be used for large-scale commercial application where economics is quite likely important.

KeywordsLactobacillus plantarum Cheese whey Nitrogen sources Response surface methodology Medium optimization AbbreviationBBDBox behnken design

DCMDry cell mass

DNSDinitrosalicyclic acid

L. LactisLactoccocus lactis

MRSde Man, Rogosa and Sharpe

RSMResponse surface methodology

Autor: Asma Manzoor - Javed Iqbal Qazi - Ikram ul Haq - Hamid Mukhtar - Akhtar Rasool


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