MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancerReportar como inadecuado




MicroRNA-205 promotes cell invasion by repressing TCF21 in human ovarian cancer - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Ovarian Research

, 10:33

First Online: 05 May 2017Received: 02 March 2017Accepted: 24 April 2017DOI: 10.1186-s13048-017-0328-1

Cite this article as: Wei, J., Zhang, L., Li, J. et al. J Ovarian Res 2017 10: 33. doi:10.1186-s13048-017-0328-1

Abstract

BackgroundOvarian cancer is the leading lethal, gynecological malignancy in the United States. No doubt, the continued morbidity and mortality of ovarian cancer reflects a poor understanding of invasive mechanisms. Recent studies reveal that ovarian cancers express aberrant microRNAs miRNAs or miRs, some of which have oncogenic or tumor suppressor properties. Several studies suggested that miR-205 is involved in tumorigenesis. Presently, we investigate the molecular mechanisms and target of miR-205 in ovarian cancer.

MethodsQuantitative real-time polymerase chain reaction and western blot were performed to assess miR-205 and transcription factor 21 TCF21 expression in ovarian cancer and normal ovary samples. The effect of miR-205 on TCF21 was determined by luciferase reporter assay and western blot. The effect of miR-205 and TCF21 on cell invasion was quantitated using transwell invasion assay.

ResultmiR-205 expression was increased in ovarian cancer and it promoted the invasive behavior of ovarian cancer cell lines OVCAR-5, OVCAR-8 and SKOV-3. miR-205 directly targeted TCF21, which was significantly decreased in ovarian cancer tissue. miR-205 inhibited TCF21 expression and as a consequence blunted the inhibitory effect of TCF21 on cell invasion. Matrix Metalloproteinases MMPs play an important role in cancer invasion and metastasis. TCF21 inhibited MMP-2 and MMP-10 and decreased ovarian cancer cell invasion. Co-transfection of TCF21 expression plasmid with miR-205 mimic diminished the inhibitory effect of TCF21 on MMP-2 and MMP-10 in ovarian cancer cells.

ConclusionmiR-205 appears to have an important role in the spread of ovarian cancer by targeting TCF21. These findings offer a new mechanism of ovarian cancer tumorigenesis, which could be useful for the development of new therapeutic approaches to ovarian cancer treatment.

KeywordsmicroRNAmiR-205 TCF21 Ovarian Cancer AbbreviationsECMExtracellular matrix

miRMicroRNA

MMPMetalloproteinase

mRNAMessenger RNA

TCF21Transcription factor 21





Autor: Jun Wei - Lahong Zhang - Jennifer Li - Shuguang Zhu - Minghui Tai - Clifford W. Mason - Julia A. Chapman - Evelyn A. Re

Fuente: https://link.springer.com/







Documentos relacionados