Genome sequence of Shigella flexneri strain SP1, a diarrheal isolate that encodes an extended-spectrum β-lactamase ESBLReportar como inadecuado

Genome sequence of Shigella flexneri strain SP1, a diarrheal isolate that encodes an extended-spectrum β-lactamase ESBL - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Annals of Clinical Microbiology and Antimicrobials

, 16:37

First Online: 12 May 2017Received: 09 February 2017Accepted: 04 May 2017DOI: 10.1186-s12941-017-0212-2

Cite this article as: Shen, P., Fan, J., Guo, L. et al. Ann Clin Microbiol Antimicrob 2017 16: 37. doi:10.1186-s12941-017-0212-2


BackgroundShigellosis is the most common cause of gastrointestinal infections in developing countries. In China, the species most frequently responsible for shigellosis is Shigella flexneri. S. flexneri remains largely unexplored from a genomic standpoint and is still described using a vocabulary based on biochemical and serological properties. Moreover, increasing numbers of ESBL-producing Shigella strains have been isolated from clinical samples. Despite this, only a few cases of ESBL-producing Shigella have been described in China. Therefore, a better understanding of ESBL-producing Shigella from a genomic standpoint is required. In this study, a S. flexneri type 1a isolate SP1 harboring blaCTX-M-14, which was recovered from the patient with diarrhea, was subjected to whole genome sequencing.

ResultsThe draft genome assembly of S. flexneri strain SP1 consisted of 4,592,345 bp with a G+C content of 50.46%. RAST analysis revealed the genome contained 4798 coding sequences CDSs and 100 RNA-encoding genes. We detected one incomplete prophage and six candidate CRISPR loci in the genome. In vitro antimicrobial susceptibility testing demonstrated that strain SP1 is resistant to ampicillin, amoxicillin-clavulanic acid, cefazolin, ceftriaxone and trimethoprim. In silico analysis detected genes mediating resistance to aminoglycosides, β-lactams, phenicol, tetracycline, sulphonamides, and trimethoprim. The blaCTX-M-14 gene was located on an IncFII2 plasmid. A series of virulence factors were identified in the genome.

ConclusionsIn this study, we report the whole genome sequence of a blaCTX-M-14-encoding S. flexneri strain SP1. Dozens of resistance determinants were detected in the genome and may be responsible for the multidrug-resistance of this strain, although further confirmation studies are warranted. Numerous virulence factors identified in the strain suggest that isolate SP1 is potential pathogenic. The availability of the genome sequence and comparative analysis with other S. flexneri strains provides the basis to further address the evolution of drug resistance mechanisms and pathogenicity in S. flexneri.

KeywordsShigella flexneri Extended-spectrum β-lactamase IncFII2 Comparative genomic analysis Electronic supplementary materialThe online version of this article doi:10.1186-s12941-017-0212-2 contains supplementary material, which is available to authorized users.

Ping Shen and Jianzhong Fan contributed equally to this article

Autor: Ping Shen - Jianzhong Fan - Lihua Guo - Jiahua Li - Ang Li - Jing Zhang - Chaoqun Ying - Jinru Ji - Hao Xu - Beiwen Zhen


Documentos relacionados