Heat pretreatment of canine samples to evaluate efficacy of imidacloprid   moxidectin and doxycycline in heartworm treatmentReportar como inadecuado

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Parasites and Vectors

, 10:246

First Online: 19 May 2017Received: 16 February 2017Accepted: 11 May 2017DOI: 10.1186-s13071-017-2189-2

Cite this article as: Bendas, A.J.R., Mendes-de-Almeida, F., Von Simson, C. et al. Parasites Vectors 2017 10: 246. doi:10.1186-s13071-017-2189-2


BackgroundConsidering the recent information on the increase of Dirofilaria immitis antigen detection by rapid assays in canine blood samples after heat treatment, the proposal that immune complexes block D. immitis antigen detection and that macrocyclic lactone + doxycycline alternative protocol might lead to increased production of those immune complexes, resulting in the erroneous diagnosis of adult worm elimination, and that there is no recommended adulticide marketed in Brazil, a study was performed to evaluate the interference of moxidectin + doxycycline moxi-doxy on diagnostic procedures when heartworm positive dogs are treated with this alternative protocol. Twenty-two naturally infected pet dogs were treated monthly with topical 10% imidacloprid + 2.5% moxidectin and with oral doxycycline 10 mg-kg BID-30 days moxi-doxy. All the dogs had their microfilaremia level determined prior to the first day of treatment, and were tested every 6 months for microfilariae Mf detection prior to heating, and for antigen detection prior to and after heating, the sample.

ResultsThe results indicate that the treatment protocol can eliminate adult heartworms as early as 6 months after the first dose, especially in low microfilaremic dogs < 300 Mf-ml. In this study, all dogs were free of heartworm antigen after 18–24 months of treatment. In a comparison of pre-heated samples and non-heated samples, sample pre-heating increased antigen detection sensitivity, and non-heated samples tended to be antigen-negative earlier than the pre-heated samples, especially when dogs had low microfilaremia levels. These discrepancies were not present in a subsequent sample of the same dog 6 months later.

ConclusionsTwo negative antigen test results 6 months apart can be recommended as the criterion to consider when a dog has been cleared of infection. The initial microfilaremia level of a dog can be used to estimate the necessary time frame to end the treatment period.

KeywordsDirofilaria immitis Heartworm Adulticide treatment Antigen detection AbbreviationsMfMicrofilariae

Moxi-doxyAlternative adulticide protocol using imidacloprid + moxidectin associated with doxycycline

Autor: Alexandre José Rodrigues Bendas - Flavya Mendes-de-Almeida - Cristiano Von Simson - Norma Labarthe

Fuente: https://link.springer.com/

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