Synergistic Effect of Mesoporous Silica and Hydroxyapatite in Loaded PolyDL-lactic-co-glycolic acid Microspheres on the Regeneration of Bone DefectsReportar como inadecuado




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BioMed Research International - Volume 2016 2016, Article ID 9824827, 14 pages -

Research Article

Department of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China

Department of Orthopaedics, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, China

Department of Orthopaedics, Tangdu Hospital, The Fourth Military Medical University, Xi’an 710038, China

Received 27 January 2016; Revised 26 June 2016; Accepted 29 June 2016

Academic Editor: Viness Pillay

Copyright © 2016 Shu He et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A microsphere composite made of polyDL-lactic-co-glycolic acid PLGA, mesoporous silica nanoparticle MSN, and nanohydroxyapatite nHA PLGA-MSN-nHA was prepared and evaluated as bone tissue engineering materials. The objective of this study was to investigate the synergistic effect of MSN-nHA on biocompatibility as well as its potential ability for bone formation. First, we found that this PLGA-MSN-nHA composite performed good characteristics on microstructure, mechanical strength, and wettability. By cell culture experiments, the adhesion and proliferation rate of the cells seeded on PLGA-MSN-nHA composite was higher than those of the controls and high levels of osteogenetic factors such as ALP and Runx-2 were detected by reverse transcriptase polymerase chain reaction. Finally, this PLGA-MSN-nHA composite was implanted into the femur bone defect in a rabbit model, and its ability to induce bone regeneration was observed by histological examinations. Twelve weeks after implantation, the bone defects had significantly more formation of mature bone and less residual materials than in the controls. These results demonstrate that this PLGA-MSN-nHA composite, introducing both MSN and nHA into PLGA microspheres, can improve the biocompatibility and osteoinductivity of composite in vitro and in vivo and had potential application in bone regeneration.





Autor: Shu He, Kai-Feng Lin, Jun-Jun Fan, Gang Hu, Xin Dong, Yi-Nan Zhao, Yue Song, Zhong-Shang Guo, Long Bi, and Jian Liu

Fuente: https://www.hindawi.com/



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