Sakuranetin Inhibits Inflammatory Enzyme, Cytokine, and Costimulatory Molecule Expression in Macrophages through Modulation of JNK, p38, and STAT1Reportar como inadecuado




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Evidence-Based Complementary and Alternative Medicine - Volume 2016 2016, Article ID 9824203, 8 pages -

Research Article

Department of Genetic Engineering, College of Life Science and Graduate School of Biotechnology, Kyung Hee University, Yongin, Republic of Korea

Graduate School of East-West Medical Science, Kyung Hee University, Yongin, Republic of Korea

Received 17 June 2016; Revised 10 August 2016; Accepted 14 August 2016

Academic Editor: Michele Navarra

Copyright © 2016 Ki-Young Kim and Hee Kang. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sakuranetin is flavonoid phytoalexin that serves as a plant antibiotic and exists in Prunus and several other plant species. Recently, we identified the anti-inflammatory effect of Prunus yedoensis and found that there were few studies on the potential anti-inflammatory activity of sakuranetin, one of the main constituents of Prunus yedoensis. Here, we isolated peritoneal macrophages from thioglycollate-injected mice and examined whether sakuranetin affected the response of the macrophages in response to lipopolysaccharide LPS plus interferon- IFN- γ or LPS only. Sakuranetin suppressed the synthesis of iNOS and COX2 in LPS-IFN-γ stimulated cells and the secretion of TNF-α, IL-6, and IL-12 in LPS stimulated cells. The surface expression of the costimulatory molecules, CD86 and CD40, was also decreased. Among the LPS-induced signaling molecules, STAT1, JNK, and p38 phosphorylation was attenuated. These findings are evidence that sakuranetin acts as anti-inflammatory flavonoid and further study is required to evaluate its in vivo efficacy.





Autor: Ki-Young Kim and Hee Kang

Fuente: https://www.hindawi.com/



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