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Enéas Gomes Filho ; José Hélio Costa ; Joaquim Enéas Filho ;Revista Ciência Agronômica 2014, 45 4

Autor: Fabrício Bonfim Sudério

Fuente: http://www.redalyc.org/


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Revista Ciência Agronômica ISSN: 0045-6888 ccarev@ufc.br Universidade Federal do Ceará Brasil Bonfim Sudério, Fabrício; Gomes Filho, Enéas; Hélio Costa, José; Filho, Joaquim Enéas B-galactosidases from cowpea stems: properties and gene expression under conditions of salt stress Revista Ciência Agronômica, vol.
45, núm.
4, octubre-diciembre, 2014, pp.
794-804 Universidade Federal do Ceará Ceará, Brasil Available in: http:--www.redalyc.org-articulo.oa?id=195331351018 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Revista Ciência Agronômica, v.
45, n.
4, p.
794-804, out-dez, 2014 Centro de Ciências Agrárias - Universidade Federal do Ceará, Fortaleza, CE www.ccarevista.ufc.br Artigo Científico ISSN 1806-6690 -galactosidases from cowpea stems: properties and gene expression under conditions of salt stress1 -galactosidases de caules de feijão-de-corda: propriedades e expressão gênica em condições de estresse salino Fabrício Bonfim Sudério2*, Enéas Gomes Filho3, José Hélio Costa4 e Joaquim Enéas Filho3 ABSTRACT - The aim of this work was to evaluate the properties and gene expression of isoforms of -galactosidase, isolated from the stems of seedlings of the cowpea cv.
Pitiuba, at different stages of development and sown in the absence and presence of NaCl.
Seeds were sown onto sheets of filter paper soaked in distilled water (control) and a 100 mM NaCl solution.
The plants were collected at the III (ED III) and VIII (ED VIII) stages of development.
Isoforms of the enzyme -gal I, II and III presented molecular weights of 89, 146 and 124 kDa respectively, and the same pH (4.0) and optimum test temperature (55 °C) under all the conditions studied.
The three isoforms were stable up to 50 °C. The thermal...





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