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Journal Title:

Diabetes

Volume:

Volume 61, Number 2

Publisher:

American Diabetes Association | 2012-02-01, Pages 542-546

Type of Work:

Article | Final Publisher PDF

Abstract: Insulin resistance IR, the hallmark of type 2 diabetes, may be under epigenetic control. This study examines the association between global DNA methylation and IR using 84 monozygotic twin pairs. IR was estimated using homeostasis model assessment HOMA. Global DNA methylation of Alu repeats in peripheral blood leukocytes was quantified by bisulfite pyrosequencing. The association between global DNA methylation and IR was examined using generalized estimating equation GEE and within-twin pair analyses, adjusting for potential confounders. Results show that methylation levels at all four CpG sites were individually associated with IR by GEE all false discovery rate-adjusted P values ≤0.026. A 10% increase in mean Alu methylation was associated with an increase of 4.55 units 95% CI 2.38-6.73 in HOMA. Intrapair difference in IR was significantly associated with intrapair difference in global methylation level. A 10% increase in the difference in mean Alu methylation was associated with an increase of 4.54 units 0.34-8.71; P = 0.036 in the difference in HOMA. Confirmation of the results by intrapair analyses suggests that genetic factors do not confound the association between global DNA methylation and IR. Exclusion of twins taking diabetes medication n = 17 did not change our results.

Subjects: Health Sciences, Epidemiology - Biology, Biostatistics - Psychology, Behavioral - Keywords: Science and Technology - Life Sciences and Biomedicine - Endocrinology and Metabolism - ENDOCRINOLOGY and METABOLISM - EPIGENETIC REGULATION - CARDIOVASCULAR-DISEASE - GENOME INSTABILITY - CPG METHYLATION - HYPERTENSION - INHERITANCE - MECHANISMS - PROMOTER - OBESITY - INFLAMMATION -



Autor: Jinying Zhao, Jack Goldberg, James Bremner, Viola Vaccarino,

Fuente: https://open.library.emory.edu/



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