Muscle contraction phenotypic analysis enabled by optogenetics reveals functional relationships of sarcomere components in Caenorhabditis elegansReportar como inadecuado




Muscle contraction phenotypic analysis enabled by optogenetics reveals functional relationships of sarcomere components in Caenorhabditis elegans - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal Title:

Scientific Reports

Volume:

Volume 6

Publisher:

Nature Publishing Group | 2016-01-29, Pages 19900-19900

Type of Work:

Article | Final Publisher PDF

Abstract: The sarcomere, the fundamental unit of muscle contraction, is a highly-ordered complex of hundreds of proteins. Despite decades of genetics work, the functional relationships and the roles of those sarcomeric proteins in animal behaviors remain unclear. In this paper, we demonstrate that optogenetic activation of the motor neurons that induce muscle contraction can facilitate quantitative studies of muscle kinetics in C. elegans. To increase the throughput of the study, we trapped multiple worms in parallel in a microfluidic device and illuminated for photoactivation of channelrhodopsin-2 to induce contractions in body wall muscles. Using image processing, the change in body size was quantified over time. A total of five parameters including rate constants for contraction and relaxation were extracted from the optogenetic assay as descriptors of sarcomere functions. To potentially relate the genes encoding the sarcomeric proteins functionally, a hierarchical clustering analysis was conducted on the basis of those parameters. Because it assesses physiological output different from conventional assays, this method provides a complement to the phenotypic analysis of C. elegans muscle mutants currently performed in many labs; the clusters may provide new insights and drive new hypotheses for functional relationships among the many sarcomere components.

Subjects: Engineering, Biomedical - Health Sciences, Pathology - Biology, Physiology - Research Funding: H.L. and G.M.B. gratefully acknowledge the financial support of Human Frontier Science Program RGP0044-2012.

Additionally, H.L. acknowledges support from the US National Institutes of Health GM088333, AG035317, and EB012803.

G.M.B. is grateful for support from NIH AR064307, and S.O. is grateful for support from NIH AR048615.

Keywords: Science and Technology - Multidisciplinary Sciences - Science and Technology - Other Topics - MYOSIN HEAVY-CHAIN - ACTIN-FILAMENTS - STRIATED-MUSCLE - PROTEIN - GENE - MUTATIONS - IDENTIFICATION - TWITCHIN - TROPONIN - PHOSPHORYLATION - Engineering - Imaging - Lab-on-a-chop - Optogenetics - Physiology -



Autor: Hyundoo Hwang, Dawn E. Barnes, Yohei Matsunaga, Guy Benian, Shoichiro Ono, Hang Lu,

Fuente: https://open.library.emory.edu/



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