Identification and spontaneous immune targeting of an endogenous retrovirus K envelope protein in the Indian rhesus macaque model of human diseaseReportar como inadecuado

Identification and spontaneous immune targeting of an endogenous retrovirus K envelope protein in the Indian rhesus macaque model of human disease - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal Title:



Volume 13, Number 1


BioMed Central | 2016-01-15, Pages 6-6

Type of Work:

Article | Final Publisher PDF

Abstract: Background: Endogenous retroviruses ERVs are remnants of ancient retroviral infections that have invaded the germ line of both humans and non-human primates. Most ERVs are functionally crippled by deletions, mutations, and hypermethylation, leading to the view that they are inert genomic fossils. However, some ERVs can produce mRNA transcripts, functional viral proteins, and even non-infectious virus particles during certain developmental and pathological processes. While there have been reports of ERV-specific immunity associated with ERV activity in humans, adaptive immune responses to ERV-encoded gene products remain poorly defined and have not been investigated in the physiologically relevant non-human primate model of human disease. Findings: Here, we identified the rhesus macaque equivalent of the biologically active human ERV-K HML-2, simian ERV-K SERV-K1, which retains intact open reading frames for both Gag and Env on chromosome 12 in the macaque genome. From macaque cells we isolated a spliced mRNA product encoding SERV-K1 Env, which possesses all the structural features of a canonical, functional retroviral Envelope protein. Furthermore, we identified rare, but robust T cell responses as well as frequent antibody responses targeting SERV-K1 Env in rhesus macaques. Conclusions: These data demonstrate that SERV-K1 retains biological activity sufficient to induce cellular and humoral immune responses in rhesus macaques. As ERV-K is the youngest and most active ERV family in the human genome, the identification and characterization of the simian orthologue in rhesus macaques provides a highly relevant animal model in which to study the role of ERV-K in developmental and disease states.

Subjects: Biology, Virology - Health Sciences, Immunology - Research Funding: This work was made possible by National Institutes of Health grants R21 AI087474, the Office of Research Infrastructure Programs P51 OD011092 and P51 OD011103, and the Bill and Melinda Gates Foundation grant 01526000084 to JBS.

Keywords: Science and Technology - Life Sciences and Biomedicine - Virology - Endogenous retroviruses ERVs - Env proteins - Antibodies - T cells - Simian immunodeficiency virus SIV - CELLS - HML-2 - EXPRESSION - INFECTION - PEPTIDES -

Autor: R. Brad Jones, Jonah B. Sacha, Helen L. Wu, Enrique J. Léon, Lyle T. Wallace, Francesca A. Nimiyongskul, Matthew B. Buechler, La



Documentos relacionados