Collapse of Cytolytic Potential in SIV-Specific CD8 T Cells Following Acute SIV Infection in Rhesus MacaquesReportar como inadecuado

Collapse of Cytolytic Potential in SIV-Specific CD8 T Cells Following Acute SIV Infection in Rhesus Macaques - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal Title:

PLoS Pathogens


Volume 12, Number 12


Public Library of Science | 2016-12-30, Pages e1006135-e1006135

Type of Work:

Article | Final Publisher PDF

Abstract: Poor maintenance of cytotoxic factor expression among HIV-specific CD8+ T cells, in part caused by dysregulated expression of the transcription factor T-bet, is associated with HIV disease progression. However, the precise evolution and context in which CD8+ T cell cytotoxic functions become dysregulated in HIV infection remain unclear. Using the rhesus macaque RM SIV infection model, we evaluated the kinetics of SIV-specific CD8+ T cell cytolytic factor expression in peripheral blood, lymph node, spleen, and gut mucosa from early acute infection through chronic infection. We identified rapid acquisition of perforin and granzyme B expression in SIV-specific CD8+ T cells in blood, secondary lymphoid tissues and gut mucosa that collapsed rapidly during the transition to chronic infection. The evolution of this expression profile was linked to low expression of T-bet and occurred independent of epitope specificity, viral escape patterns and tissue origin. Importantly, during acute infection SIV-specific CD8+ T cells that maintained T-bet expression retained the ability to express granzyme B after stimulation, but this relationship was lost in chronic infection. Together, these data demonstrate the loss of cytolytic machinery in SIV-specific CD8+ T cells in blood and at tissue sites of viral reservoir and active replication during the transition from acute to chronic infection. This phenomenon occurs despite persistent high levels of viremia suggesting that an inability to maintain properly regulated cytotoxic T cell responses in all tissue sites enables HIV-SIV to avoid immune clearance, establish persistent viral reservoirs in lymphoid tissues and gut mucosa, and lead ultimately to immunopathogenesis and death.

Subjects: Health Sciences, Immunology - Biology, Microbiology - Research Funding: Funding for this study was provided by National Institutes of Health R56 AI106481 and granted to MRB and GS.

Additional funding from the National Institutes of Health Office of Research Infrastructure Programs Grant OD P51OD011132 to the Yerkes National Primate Research Center, as well as resources from Emory Center for AIDS Research, National Institutes of Health grant P30-50409, and the University of Pennsylvania Center for AIDS Research, National Institutes of Health Grant P30 A145008.

Resources for viral sequencing were granted to GMS from the National Institutes of HealthGrants R56 AI118549 and R33 AI087383.

Keywords: CD8+ T-CELLS - SIV INFECTION - Immunology - Rhesus macaque -

Autor: Emily R. Roberts, Diane G. Carnathan, Hui Li, Guido Shaw, Guido Silvestri, Michael R. Betts,



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