Abundance and Significance of Neuroligin-1 and Neurexin II in the Enteric Nervous System of Embryonic RatsReportar como inadecuado




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BioMed Research International - Volume 2017 2017, Article ID 1209360, 6 pages - https:-doi.org-10.1155-2017-1209360

Research Article

Department of Pediatric Surgery, Qilu Hospital, Shandong University, Shandong, China

Internal Medicine, Qingzhou Clinical School, Weifang Medical College, Weifang, China

Department of Pediatric Surgery, Weihai Municipal Hospital, Shandong, China

Correspondence should be addressed to Aiwu Li

Received 23 September 2016; Revised 19 December 2016; Accepted 4 January 2017; Published 18 January 2017

Academic Editor: Richard Tucker

Copyright © 2017 Dongming Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. To investigate the abundance of neuroligin-1 and neurexin II in the enteric nervous system ENS of rats on different embryonic days and to explore their potential significance. Methods. The full-thickness colon specimens proximal to the ileocecal junction of rats on embryonic days 16, 18, and 20 and of newborns within 24 hours E16, E18, E20, and Ep0 were studied, respectively. qRT-PCR was applied for detecting the expressions of neuroligin-1 and neurexin II on mRNA, and western blotting was employed for detecting their further expressions on the whole tissue. Finally, the histological appearance of neuroligin-1 and neurexin IIα was elucidated using immunohistochemical staining. Results. qRT-PCR showed that the neuroligin-1 and neurexin II mRNA expressions of groups E16, E18, E20, and Ep0 increased gradually with the growth of embryonic rats . Western blotting confirmed the increasing tendency. In immunohistochemical staining, proteins neuroligin-1 and neurexin IIα positive cells concentrated mostly in the myenteric nerve plexus of the colon and their expressions depend on the embryonic time. Conclusion. Neuroligin-1 and neurexin II were both expressed in the ENS and have temporal correlation with the development of ENS, during which neuronal intestinal malformations NIM may occur due to their disruptions and consequent abnormal ENS development.





Autor: Dongming Wang, Jingnian Pan, Guoxin Song, Ni Gao, Yi Zheng, Qiangye Zhang, and Aiwu Li

Fuente: https://www.hindawi.com/



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