RNA Vaccination Therapy: Advances in an Emerging FieldReportar como inadecuado

RNA Vaccination Therapy: Advances in an Emerging Field - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Immunology Research - Volume 2016 2016, Article ID 9703914, 2 pages -


TRON-Translational Oncology at the University Medical Center of the Johannes Gutenberg University gGmbH, 55131 Mainz, Germany

Department of Dermatology, University Hospital of Zurich, 8091 Zurich, Switzerland

Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA

Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA

Laboratory of Molecular and Cellular Therapy LMCT, Vrije Universiteit Brussel VUB, 1090 Brussels, Belgium

Avidity NanoMedicines, La Jolla, CA 92037, USA

Received 27 January 2016; Accepted 28 January 2016

Copyright © 2016 Sebastian Kreiter et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

After more than two decades of research, the efforts to translate the concept of RNA based vaccination have reached a critical mass. Several preclinical and clinical projects located in the academic or industrial setting are underway and the coming years will allow us to get broad insight into clinical feasibility, safety, and first efficacy data. It can be anticipated that some RNA based vaccines will be approved within the near future.

The use of in vitro transcribed RNA is now viewed as an attractive approach for vaccination therapies, with several features contributing to its favorable characteristics. RNA allows expression of molecularly well-defined proteins and its half-life can be steered through modifications in the RNA backbone. Moreover, unlike DNA, RNA does not need to enter the nucleus during transfection and there is no risk of integration into the genome, assuring safety through transient activity. Rapid design and synthesis in response to demand, accompanied by inexpensive pharmaceutical production, are additional features facilitating its clinical translation.

The seminal work of Wolff et al. which showed that RNA injected directly into skeletal muscle can lead to protein expression opened the era of RNA based therapeutics 1. This observation was followed by Martinon et al. and Conry et al. who performed the first vaccinations with viral- and cancer-antigen encoding RNA, respectively, and elicited antigen-specific immune responses 2, 3. RNA based vaccination was also carried out by ex vivo transfection of mRNA into autologous dendritic cells DCs which was initially described by Boczkowski et al. 4. Along with the introduction of highly efficient transfection methods for RNA 5, several preclinical and clinical studies showed the safety and efficacy of this RNA based vaccination strategy 6. In a different setting, Hoerr et al. proved that direct injection of naked or protamine-protected RNA intradermally can lead to induction of T cell and antibody responses in preclinical models and then translated the approach into a clinical setting 7–10. Personalized cancer vaccination with RNA and intravenous delivery of liposome-complexed RNA 11, 12 are other recent promising strategies that have reached the clinical stage. In addition to cancer, other disease settings such as infectious diseases as well as allergy were also shown to benefit from RNA based vaccination 13–15.

In this special issue, a number of papers will illustrate and summarize the advances in this emerging field. M. A. McNamara et al. will provide a comprehensive review on RNA based vaccines in cancer immunotherapy, which is further detailed for the use of mutanome engineered RNA by M. Vormehr et al. These will be complemented by a review from K. K. L. Phua describing targeted delivery systems for RNA based nanoparticle tumor vaccines. Other contributions will describe RNA based methods for in vitro analytics such as cytotoxicity T. A. Omokoko et al. or effects of RNA on transcriptome of DCs S. Hoyer et al

Finally, E. Hattinger et al. will also demonstrate, with a different disease focus, the efficacy of prophylactic RNA vaccination against allergy.

In conclusion, this special issue covers many aspects of RNA based vaccines. As RNA based vaccination is not the only application of the RNA technology RNA based protein replacement, immunomodulation, and cellular therapy are further promising fields of development, we hope to have sparked the readers interest in RNA based therapies in general.

Sebastian KreiterMustafa DikenSteve PascoloSmita K. NairKris M. ThielemansAndrew Geall


J. A. Wolff, R. W. Malone, P. Williams et al. -Direct gene transfer into mouse muscle in vivo,- Science, vol. 247, no. 4949, pp. 1465–1468, 1990. View at Publisher · View at Google Scholar · View at ScopusF. Martinon, S. Krishnan, G. Lenzen et al. -Induction of virus-specific cytotoxic T lymphocytes in vivo by liposome-entrapped mRNA,- European Journal of Immunology, vol. 23, no. 7, pp. 1719–1722, 1993. View at Publisher · View at Google Scholar · View at ScopusR. M. Conry, A. F. LoBuglio, M. Wright et al. -Characterization of a messenger RNA polynucleotide vaccine vector,- Cancer Research, vol. 55, no. 7, pp. 1397–1400, 1995. View at Google Scholar · View at ScopusD. Boczkowski, S. K. Nair, D. Snyder, and E. Gilboa -Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo,- The Journal of Experimental Medicine, vol. 184, no. 2, pp. 465–472, 1996. View at Publisher · View at Google Scholar · View at ScopusA. Bonehill, A. M. T. Van Nuffel, J. Corthals et al. -Single-step antigen loading and activation of dendritic cells by mRNA electroporation for the purpose of therapeutic vaccination in melanoma patients,- Clinical Cancer Research, vol. 15, no. 10, pp. 3366–3375, 2009. View at Publisher · View at Google Scholar · View at ScopusD. Benteyn, C. Heirman, A. Bonehill, K. Thielemans, and K. Breckpot -mRNA-based dendritic cell vaccines,- Expert Review of Vaccines, vol. 14, no. 2, pp. 161–176, 2015. View at Publisher · View at Google Scholar · View at ScopusI. Hoerr, R. Obst, H.-G. Rammensee, and G. Jung -In vivo application of RNA leads to induction of specific cytotoxic T lymphocytes and antibodies,- European Journal of Immunology, vol. 30, no. 1, pp. 1–7, 2000. View at Publisher · View at Google Scholar · View at ScopusB. Weide, J.-P. Carralot, A. Reese et al. -Results of the first phase I-II clinical vaccination trial with direct injection of mRNA,- Journal of Immunotherapy, vol. 31, no. 2, pp. 180–188, 2008. View at Publisher · View at Google Scholar · View at ScopusB. Weide, S. Pascolo, B. Scheel et al. -Direct injection of protamine-protected mRNA: results of a phase 1-2 vaccination trial in metastatic melanoma patients,- Journal of Immunotherapy, vol. 32, no. 5, pp. 498–507, 2009. View at Publisher · View at Google Scholar · View at ScopusS. M. Rittig, M. Haentschel, K. J. Weimer et al. -Intradermal vaccinations with RNA coding for TAA generate CD8

and CD4

immune responses and induce clinical benefit in vaccinated patients,- Molecular Therapy, vol. 19, no. 5, pp. 990–999, 2011. View at Publisher · View at Google Scholar · View at ScopusJ. C. Castle, S. Kreiter, J. Diekmann et al. -Exploiting the mutanome for tumor vaccination,- Cancer Research, vol. 72, no. 5, pp. 1081–1091, 2012. View at Publisher · View at Google Scholar · View at ScopusS. Kreiter, M. Vormehr, N. van de Roemer et al. -Mutant MHC class II epitopes drive therapeutic immune responses to cancer,- Nature, vol. 520, no. 7549, pp. 692–696, 2015. View at Publisher · View at Google ScholarB. Petsch, M. Schnee, A. B. Vogel et al. -Protective efficacy of in vitro synthesized, specific mRNA vaccines against influenza A virus infection,- Nature Biotechnology, vol. 30, no. 12, pp. 1210–1216, 2012. View at Publisher · View at Google Scholar · View at ScopusM. Brazzoli, D. Magini, A. Bonci et al. -Induction of broad-based immunity and protective efficacy by self-amplifying mRNA vaccines encoding influenza virus hemagglutinin,- Journal of Virology, vol. 90, no. 1, pp. 332–344, 2015. View at Publisher · View at Google ScholarR. Weiss, S. Scheiblhofer, E. Roesler, E. Weinberger, and J. Thalhamer -MRNA vaccination as a safe approach for specific protection from type I allergy,- Expert Review of Vaccines, vol. 11, no. 1, pp. 55–67, 2012. View at Publisher · View at Google Scholar · View at Scopus

Autor: Sebastian Kreiter, Mustafa Diken, Steve Pascolo, Smita K. Nair, Kris M. Thielemans, and Andrew Geall

Fuente: https://www.hindawi.com/


Documentos relacionados