Protective Effects of Streblus asper Leaf Extract on H2O2-Induced ROS in SK-N-SH Cells and MPTP-Induced Parkinson’s Disease-Like Symptoms in C57BL-6 MouseReportar como inadecuado




Protective Effects of Streblus asper Leaf Extract on H2O2-Induced ROS in SK-N-SH Cells and MPTP-Induced Parkinson’s Disease-Like Symptoms in C57BL-6 Mouse - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Evidence-Based Complementary and Alternative Medicine - Volume 2015 2015, Article ID 970354, 6 pages -

Research ArticleDepartment of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand

Received 15 October 2015; Accepted 7 December 2015

Academic Editor: Giuseppe Esposito

Copyright © 2015 Kanathip Singsai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigated the effects of Streblus asper leaf extract SA on reactive oxygen species ROS in SK-N-SH cell culture and on motor functions and behaviors in MPTP-treated C57BL-6 mice. SK-N-SH cell viability after incubation with SA for 24 h was measured by MTT assay. Intracellular ROS levels of SK-N-SH cells were quantified after pretreatment with SA 0, 200, 600, and 1000 µg-mL in the presence of H2O2 300 µM. Male C57BL-6 mice were force-fed with water or 200 mg-kg-day SA for 32 days. Intraperitoneal injection of MPTP was used to induce Parkinson’s disease-like symptoms. Catalepsy, beam balance ability, olfactory discrimination, social recognition, and spontaneous locomotor activity were assessed on days 19, 21, 23, 26, and 32, respectively. In cell culture, SA at 200, 600, and 1000 µg-mL significantly decreased ROS levels in H2O2-treated SK-N-SH cells. MPTP-treated C57BL-6 mice showed a significant change in all parameters tested when compared to the control group. Pretreatment and concurrent treatment with 200 mg-kg-day SA could antagonize the motor and cognitive function deficits induced by MPTP. The results show that SA possesses anti-Parkinson effects in MPTP-treated C57BL-6 mice and that reduction in ROS levels might be one of the mechanisms.





Autor: Kanathip Singsai, Tarinee Akaravichien, Veerapol Kukongviriyapan, and Jintana Sattayasai

Fuente: https://www.hindawi.com/



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