Comparative mRNA Expression of eEF1A Isoforms and a PI3K-Akt-mTOR Pathway in a Cellular Model of Parkinson’s DiseaseReportar como inadecuado

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Parkinson’s Disease - Volume 2016 2016, Article ID 8716016, 11 pages -

Research ArticleDepartment of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

Received 14 October 2015; Revised 23 December 2015; Accepted 19 January 2016

Academic Editor: Hélio Teive

Copyright © 2016 Kawinthra Khwanraj et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The PI3K-Akt-mTOR pathway is one of dysregulated pathways in Parkinson’s disease PD. Previous studies in nonneuronal cells showed that Akt regulation can be increased by eukaryotic protein elongation factor 1 alpha 2 eEF1A2. eEF1A2 is proposed to contribute protection against apoptotic death, likely through activation of the PI3K-Akt pathway. Whether eEF1A2 plays a role in the prevention of cell death in PD has not been investigated. Recently, gene profiling on dopaminergic neurons from postmortem PD patients showed both upregulation and downregulation of some PI3K and mTOR genes. In this paper, the expression of all gene members of the PI3K-Akt-mTOR pathway in relation to those of the eEF1A isoforms in a cellular model of PD was investigated at the mRNA level. The results showed a similar trend of upregulation of genes of the eEF1A isoforms eEF1A1 and eEF1A2 and of the PI3K classes I–III-Akt Akt1, Akt2, and Akt3-mTOR mTORC1 and mTORC2 pathway in both nondifferentiated and differentiated SH-SY5Y dopaminergic cells treated with 1-methyl-4-phenylpyridinium MPP

. Upregulation of eEF1A2, Akt1, and mTORC1 was consistent with the relative increase of eEF1A2, Akt, phospho-Akt, and mTORC1 proteins. The possible role of eEF1A isoforms in the regulation of the PI3K-Akt-mTOR pathway in PD is discussed.

Autor: Kawinthra Khwanraj, Suriyat Madlah, Khwanthana Grataitong, and Permphan Dharmasaroja



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