Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells In VitroReportar como inadecuado




Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells In Vitro - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Stem Cells International - Volume 2017 2017, Article ID 8371326, 10 pages - https:-doi.org-10.1155-2017-8371326

Research Article

Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, Japan

Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Japan

Department of Oral Pathology, Nihon University School of Dentistry at Matsudo, Matsudo, Japan

Correspondence should be addressed to Toshirou Kondoh

Received 5 September 2016; Accepted 28 December 2016; Published 5 February 2017

Academic Editor: Andrzej Lange

Copyright © 2017 Shingo Kanao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The dental follicle is an ectomesenchymal tissue surrounding the developing tooth germ. Human dental follicle cells hDFCs have the capacity to commit to differentiation into multiple cell types. Here we investigated the capacity of hDFCs to differentiate into neural cells and the efficiency of a two-step strategy involving floating neurosphere-like bodies for neural differentiation. Undifferentiated hDFCs showed a spindle-like morphology and were positive for neural markers such as nestin, β-III-tubulin, and S100β. The cellular morphology of several cells was neuronal-like including branched dendrite-like processes and neurites. Next, hDFCs were used for neurosphere formation in serum-free medium containing basic fibroblast growth factor, epidermal growth factor, and B27 supplement. The number of cells with neuronal-like morphology and that were strongly positive for neural markers increased with sphere formation. Gene expression of neural markers also increased in hDFCs with sphere formation. Next, gene expression of neural markers was examined in hDFCs during neuronal differentiation after sphere formation. Expression of Musashi-1 and Musashi-2, MAP2, GFAP, MBP, and SOX10 was upregulated in hDFCs undergoing neuronal differentiation via neurospheres, whereas expression of nestin and β-III-tubulin was downregulated. In conclusion, hDFCs may be another optimal source of neural-glial cells for cell-based therapies to treat neurological diseases.





Autor: Shingo Kanao, Naomi Ogura, Kosuke Takahashi, Ko Ito, Masaaki Suemitsu, Kayo Kuyama, and Toshirou Kondoh

Fuente: https://www.hindawi.com/



DESCARGAR PDF




Documentos relacionados