Pandemic extra-intestinal pathogenic Escherichia coli ExPEC clonal group O6-B2-ST73 as a cause of avian colibacillosis in BrazilReportar como inadecuado

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Extra-intestinal pathogenic Escherichia coli ExPEC represent an emerging pathogen, with pandemic strains increasingly involved in cases of urinary tract infections UTIs, bacteremia, and meningitis. In addition to affecting humans, the avian pathotype of ExPEC, avian pathogenic E. coli APEC, causes severe economic losses to the poultry industry. Several studies have revealed overlapping characteristics between APEC and human ExPEC, leading to the hypothesis of a zoonotic potential of poultry strains. However, the description of certain important pandemic clones, such as Sequence Type 73 ST73, has not been reported in food sources. We characterized 27 temporally matched APEC strains from diverse poultry farms in Brazil belonging to the O6 serogroup because this serogroup is frequently described as a causal factor in UTI and septicemia in humans in Brazil and worldwide. The isolates were genotypically characterized by identifying ExPEC virulence factors, phylogenetically tested by phylogrouping and multilocus sequence type MLST analysis, and compared to determine their similarity employing the pulsed field gel electrophoresis PFGE technique. The strains harbored a large number of virulence determinants that are commonly described in uropathogenic E. coli UPEC and sepsis associated E. coli SEPEC strains and, to a lesser extent in neonatal meningitis associated E. coli NMEC, such as pap 85%, sfa 100%, usp 100%, cnf1 22%, kpsMTII 66%, hlyA 52%, and ibeA 4%. These isolates also yielded a low prevalence of some genes that are frequently described in APEC, such as iss 37%, tsh, ompT, and hlyF 8% each, and cvi-cva 0%. All strains were classified as part of the B2 phylogroup and sequence type 73 ST73, with a cluster of 25 strains showing a clonal profile by PFGE. These results further suggest the zoonotic potential of some APEC clonal lineages and their possible role in the epidemiology of human ExPEC, in addition to providing the first description of the O6-B2-ST73 clonal group in poultry.

Autor: Marcos Paulo Vieira Cunha, Andre Becker Saidenberg, Andrea Micke Moreno, Antonio José Piantino Ferreira, Mônica Aparecida Midol



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