A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysisReportar como inadecuado




A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysis - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Amplicon targeted sequencing by massively parallel sequencing PCR-MPS is a potential method for use in forensic DNA analyses. In this application, PCR-MPS may supplement or replace other instrumental analysis methods such as capillary electrophoresis and Sanger sequencing for STR and mitochondrial DNA typing, respectively. PCR-MPS also may enable the expansion of forensic DNA analysis methods to include new marker systems such as single nucleotide polymorphisms SNPs and insertion-deletions indels that currently are assayable using various instrumental analysis methods including microarray and quantitative PCR. Acceptance of PCR-MPS as a forensic method will depend in part upon developing protocols and criteria that define the limitations of a method, including a defensible analytical threshold or method detection limit. This paper describes an approach to establish objective analytical thresholds suitable for multiplexed PCR-MPS methods. A definition is proposed for PCR-MPS method background noise, and an analytical threshold based on background noise is described.



Autor: Brian Young , Jonathan L. King , Bruce Budowle , Luigi Armogida

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados