Chemical and structural characterization of a model Post-Termination Complex PoTC for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-GReportar como inadecuado




Chemical and structural characterization of a model Post-Termination Complex PoTC for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-G - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

A model Post-Termination Complex PoTC used for the discovery of Ribosome Recycling Factor RRF was purified and characterized by cryo-electron microscopic analysis and biochemical methods. We established that the model PoTC has mostly one tRNA, at the P-E or P-P position, together with one mRNA. The structural studies were supported by the biochemical measurement of bound tRNA and mRNA. Using this substrate, we establish that the release of tRNA, release of mRNA and splitting of ribosomal subunits occur during the recycling reaction. Order of these events is tRNA release first followed by mRNA release and splitting almost simultaneously. Moreover, we demonstrate that IF3 is not involved in any of the recycling reactions but simply prevents the re-association of split ribosomal subunits. Our finding demonstrates that the important function of RRF includes the release of mRNA, which is often missed by the use of a short ORF with the Shine-Dalgarno sequence near the termination site.



Autor: Nobuhiro Iwakura, Takeshi Yokoyama, Fabio Quaglia, Kaoru Mitsuoka, Kazuhiro Mio, Hideki Shigematsu, Mikako Shirouzu, Akira Kaji ,

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados