Molecular characterization of mudskipper Boleophthalmus pectinirostris hypoxia-inducible factor-1α HIF-1α and analysis of its function in monocytes-macrophagesReportar como inadecuado




Molecular characterization of mudskipper Boleophthalmus pectinirostris hypoxia-inducible factor-1α HIF-1α and analysis of its function in monocytes-macrophages - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Hypoxia-inducible factor-1α HIF-1α plays a critical role in immune and inflammatory responses and is important in controlling a variety of processes in monocytes and macrophages. However, very little information is available about the functions of HIF-1α in fish monocytes-macrophages MO-MФ. In this study, the cDNA sequence of the mudskipper Boleophthalmus pectinirostris HIF-1α gene BpHIF-1α was determined. Sequence comparison and phylogenetic tree analysis showed that BpHIF-1α is clustered in the fish HIF-1α tree. Constitutive expression of BpHIF-1α mRNA was detected by real-time quantitative PCR in all tested tissues, and the expression was found to be dramatically increased in the skin, liver, spleen, and kidney after Edwardsiella tarda infection. In addition, hypoxia and infection induced the expression of the BpHIF-1α transcript and protein in MO-MФ, respectively. Hypoxia caused an increase in phagocytic and bactericidal capacity of mudskipper MO-MФ in a BpHIF-1α-dependent manner. BpHIF-1α induced an anti-inflammatory status in MO-MФ upon E. tarda infection and hypoxia. Therefore, BpHIF-1α may play a predominant role in the modulation of mudskipper MO-MФ function in the innate immune system.



Autor: Feng Guan , Xin-Jiang Lu , Chang-Hong Li, Jiong Chen

Fuente: http://plos.srce.hr/



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