Screening test for neutralizing antibodies against yellow fever virus, based on a flavivirus pseudotypeReport as inadecuate




Screening test for neutralizing antibodies against yellow fever virus, based on a flavivirus pseudotype - Download this document for free, or read online. Document in PDF available to download.

Given the possibility of yellow fever virus reintroduction in epidemiologically receptive geographic areas, the risk of vaccine supply disruption is a serious issue. New strategies to reduce the doses of injected vaccines should be evaluated very carefully in terms of immunogenicity. The plaque reduction test for the determination of neutralizing antibodies PRNT is particularly time-consuming and requires the use of a confinement laboratory. We have developed a new test based on the use of a non-infectious pseudovirus WN-YF17D. The presence of a reporter gene allows sensitive determination of neutralizing antibodies by flow cytometry. This WN-YF17D test was as sensitive as PRNT for the follow-up of yellow fever vaccinees. Both tests lacked specificity with sera from patients hospitalized for acute Dengue virus infection. Conversely, both assays were strictly negative in adults never exposed to flavivirus infection or vaccination, and in patients sampled some time after acute Dengue infection. This WN-YF17D test will be particularly useful for large epidemiological studies and for screening for neutralizing antibodies against yellow fever virus.



Author: Séverine Mercier-Delarue, Christine Durier, Nathalie Colin de Verdière, Jean-Dominique Poveda, Vincent Meiffrédy, Maria Dolore

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents