Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumoniaReport as inadecuate




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Mycoplasma pneumoniae M. pneumoniae infection can cause community acquired pneumonia in children. A real-time method of simultaneous amplification and testing of M. pneumoniae SAT-MP was developed to diagnose M. pneumoniae targeting a region of the ribosomal RNA. The SAT-MP assay can accurately identify M. pneumoniae with a detection range from 101 to 107 CFU-ml. In this study, the specimens from 315 children with pneumonia were collected and analyzed by SAT-MP in parallel with real-time PCR method and IgM ELISA assay. The positive rates of these specimens examined by SAT-MP assay, real-time PCR method and IgM ELISA assay were 16.51%, 15.56% and 12.70% respectively. While there was statistical significance p = 0.04 between SAT-MP assay and IgM ELISA assay, no statistical significance p = 0.25 was found between SAT-MP assay and real-time PCR method and these two methods had high consistency kappa value = 0.97. These findings indicate that the newly developed SAT-MP assay is a rapid, sensitive and specific method for identifying M. pneumoniae with potential clinical application in the early diagnosis of M. pneumoniae infection.



Author: Wei Li, You-hong Fang, Hong-qiang Shen, De-hua Yang, Qiang Shu, Shi-qiang Shang

Source: http://plos.srce.hr/



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