The effect of biological sealants and adhesive treatments on matrix metalloproteinase expression during renal injury healingReport as inadecuate




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Background

Renal injuries are relatively common in cases of abdominal trauma. Adhesives and sealants can be used to repair and preserve damaged organs. Using a rat model, this study explores the activity of different matrix metalloproteinases MMP during the healing of renal injuries treated by two biological adhesives TachoSil and GelitaSpon and a new synthetic elastic cyanoacrylate Adhflex.

Methods

Renal traumatic injuries were experimentally induced in 90 male Wistar rats by a Stiefel Biopsy Punch in the anterior aspect of the left kidney. Animals were divided into five groups: 1, sham non-injured n = 3; 2, non-treated standard punch injury n = 6; 3, punch injury treated with TachoSil n = 27; 4, punch injury treated with GelitaSpon n = 27; and, 5, punch injury treated with Adhflex n = 27. Wound healing was evaluated 2, 6, and 18 days after injury by determining the expression of MMPs, and the histopathological evolution of lesions.

Findings

Histologically, the wound size at 6 days post-injury was larger in Adhflex-treated samples than in the other treatments, but the scarring tissue was similar at 18 days post-injury. Only the MMPs subtypes 1, 2, 8, 9, and 13 were sufficiently expressed to be quantifiable. Both time since injury and treatment type had a significant influence on MMPs expression. Two days after injury, the expression of MMP8 and MMP9 was predominant. MMP2 expression was greater 6 days after injury. The Adhflex-treated group had a significantly higher MMPs expression than the other treatment groups at all healing stages.

Conclusions

All three sealant treatments induced almost similar expression of MMPs than untreated animals indicating a physiological healing process. Given that all renal trauma injuries must be considered emergencies, both biological and synthetic adhesives, such as Adhflex, should be considered as a treatment options.



Author: José Miguel Lloris-Carsí , Carlos Barrios , Beatriz Prieto-Moure , José Miguel Lloris-Cejalvo , Dolores Cejalvo-Lapeña

Source: http://plos.srce.hr/



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