Sphingolipids as a new factor in the pathomechanism of preeclampsia – Mass spectrometry analysisReport as inadecuate




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Objectives and design

The aim of the study was to analyse a panel of 11 sphingolipids in plasma and three blood fractions platelet-poor plasma, platelets and red blood cells of women with mild preeclampsia.

Materials and methods

We recruited 21 women between 25–40 weeks gestation with diagnosed mild preeclampsia to the study group and 36 healthy women with uncomplicated pregnancies, who corresponded with the study group according to gestational age, to the control group. To assess the concentration of 11 sphingolipids in the blood plasma and blood fractions, we used ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry UHPLC-MS-MS.

Results

We showed a significant increase in the concentration of eight sphingolipids in the plasma of women with preeclampsia in comparison to the control group: Sph p = 0.0032, S1P p = 0.0289, C20-Cer p < 0.0001, C18-Cer p < 0.0001, C16-Cer p = 0.012, C18:1-Cer p = 0.003, C22-Cer p = 0.0071, and C24:1-Cer p = 0.0085.

Conclusion

We showed that selected sphingolipids, especially C20-Cer and C18-Cer, are totally new factors in the pathomechanism of PE and that these bioactive lipids may play an important role in apoptosis and autophagy.



Author: Karol Charkiewicz, Joanna Goscik, Agnieszka Blachnio-Zabielska, Grzegorz Raba, Agata Sakowicz, Jaroslaw Kalinka, Adrian Chabowski

Source: http://plos.srce.hr/



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