Sequencing of small RNAs of the fern Pleopeltis minima Polypodiaceae offers insight into the evolution of the microrna repertoire in land plantsReport as inadecuate




Sequencing of small RNAs of the fern Pleopeltis minima Polypodiaceae offers insight into the evolution of the microrna repertoire in land plants - Download this document for free, or read online. Document in PDF available to download.

MicroRNAs miRNAs are short, single stranded RNA molecules that regulate the stability and translation of messenger RNAs in diverse eukaryotic groups. Several miRNA genes are of ancient origin and have been maintained in the genomes of animal and plant taxa for hundreds of millions of years, playing key roles in development and physiology. In the last decade, genome and small RNA sRNA sequencing of several plant species have helped unveil the evolutionary history of land plants. Among these, the fern group monilophytes occupies a key phylogenetic position, as it represents the closest extant cousin taxon of seed plants, i.e. gymno- and angiosperms. However, in spite of their evolutionary, economic and ecological importance, no fern genome has been sequenced yet and few genomic resources are available for this group. Here, we sequenced the small RNA fraction of an epiphytic South American fern, Pleopeltis minima Polypodiaceae, and compared it to plant miRNA databases, allowing for the identification of miRNA families that are shared by all land plants, shared by all vascular plants tracheophytes or shared by euphyllophytes ferns and seed plants only. Using the recently described transcriptome of another fern, Lygodium japonicum, we also estimated the degree of conservation of fern miRNA targets in relation to other plant groups. Our results pinpoint the origin of several miRNA families in the land plant evolutionary tree with more precision and are a resource for future genomic and functional studies of fern miRNAs.



Author: Florencia Berruezo, Flávio S. J. de Souza, Pablo I. Picca, Sergio I. Nemirovsky, Leandro Martínez Tosar, Mercedes Rivero, Aleja

Source: http://plos.srce.hr/



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