Genetic polymorphism of Merozoite Surface Protein-2 MSP-2 in Plasmodium falciparum isolates from Pawe District, North West EthiopiaReport as inadecuate




Genetic polymorphism of Merozoite Surface Protein-2 MSP-2 in Plasmodium falciparum isolates from Pawe District, North West Ethiopia - Download this document for free, or read online. Document in PDF available to download.

Background

In malaria endemic regions, Plasmodium falciparum infection is characterized by extensive genetic diversity. Describing this diversity provides important information about the local malaria situation. This study was conducted to evaluate the extent of genetic diversity of P. falciparum in Pawe district, North West Ethiopia, using the highly polymorphic merozoite surface protein 2 gene.

Methods

Atotal of 92 isolates from patients with uncomplicated P. falciparum attending Pawe Health Centre were collected from September to December 2013. Genomic DNA was extracted using the Chelex method and analyzed by length polymorphism following gel electrophoresis of DNA products from nested PCR of msp2 block 3, targeting allelic families of FC27 and 3D7-IC.

Results

There were twenty-two different msp2 alleles, 11 corresponding to the 3D7- IC and 11 to the FC27 allelic family. The frequency of isolates of the msp2 3D7-IC allelic familywas higher 51% compared to FC27 49%. The majority of the isolates 76% contained multiple infections andthe overall mean multiplicity of infection was 2.8 CI 95% 2.55–3.03. The heterozygosity index was 0.66 for msp2. There was no statically significant difference in the multiplicity of infection by age or parasite density.

Conclusions

The results of this study show that P.falciparum polymorphismsare extensive in Northwest Ethiopia and most of the infections are composed of multiple clones.



Author: Hussein Mohammed , Moges Kassa, Ashenafi Assefa, Mekonnen Tadesse, Amha Kebede

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents